Fig 1: METTL1 and WDR4 are upregulated and associated with poor prognosis of HNSCC patients. (A) TCGA data showed that METTL1 (left) and WDR4 (right) were significantly upregulated in HNSCC tissues (n = 502) as compared with normal tissues (n = 44). (B) The correlation between METTL1 and WDR4 levels in HNSCC tissues using TCGA data. (C) Kaplan-Meier curves of overall survival based on METTL1 (left) and WDR4 (right) levels in HNSCC tissues from TCGA data. (D) Representative images of IHC staining for METTL1 (left) and quantification (right) between 140 HNSCC tissue samples and 69 normal tissue samples. (E) Representative images of IHC staining for WDR4 (left) and quantification (right) between 140 HNSCC tissue samples and 69 normal tissue samples. (F) Kaplan-Meier curves of overall survival based on METTL1 (left) and WDR4 (right) levels in HNSCC patients from our cohort. (G) The correlation between METTL1 and WDR4 levels in HNSCC patients from our cohort. (H,I) The mRNA levels and protein levels of METTL1 and WDR4 and m7G tRNA modification were upregulated in human HNSCC specimens examined by qRT-PCR (H), Western blotting and Northwestern blotting (I). T1/T2 and N1/N2 represented tumor tissues and surrounding normal mucosal tissues from two HNSCC patients, respectively. Data are presented as the mean ± SD and analyzed by Student's t-test, log rank test, or Pearson's correlation test. **, P < 0.01, ***, P < 0.001. Abbreviations: METTL1: Methyltransferase-like 1; WDR4: WD repeat domain 4; HNSCC: head and neck squamous cell carcinoma; TCGA: the Cancer Genome Atlas; IHC: immunohistochemistry; m7G: N7-methylguanosine; qRT-PCR: quantitative real-time PCR
Fig 2: METTL1 is upregulated in prostate cancer. A A schematic overview of the research workflow used to identify altered expression of RMPs associated with PCa. B Heatmap of average Z-scores of mRNA expression values in human primary (P) and metastatic (M) PCa samples compared to healthy tissue for significant differentially expressed RNA-modifying enzymes reveals METTL1 as the most overexpressed RMPs in PCa. Data from are from Grasso et al. [7] (n = N: 12, P: 49, M: 27); Taylor et al. [6] (n = N: 29, P: 131, M: 19); Varambally et al. [42] (n = N: 6, P: 7, M: 6); Lapointe et al. [44] (n = N: 9, P: 13, M: 4); Tomlins et al. [43] (n = N: 23, P: 32, M: 20) (left panel) datasets. Z-score averages for all datasets are also shown as “Aver”. Z-score values are indicated using numeric values. Grey Z-score values indicate no significant p-value. The right heatmap shows the average log2 fold change in mRNA expression values in Pten-cKO mice with prostate intraepithelial neoplasia (PIN) and invasive prostate carcinoma (Inv) compared to normal prostate tissue (right panel) (n = 4). C METTL1 and WDR4 expression are increased in primary (PT) and metastatic tumours (M) compared to normal tissues (N). Data are from Grasso et al. [7], Taylor et al. [6], and Varambally et al. [42] datasets. Log2-normalised gene expression values are shown. D High expression of METTL1 but not WDR4 is associated with poor patient prognosis. Kaplan–Meier curves representing biochemical recurrence-free survival (DFS) of patient groups selected according to gene expression, data from the Cambridge, Stockholm and Taylor cohorts [6, 46]. Data were retrieved from the camcAPP [48] and cBioportal. E Western blot from benign prostatic hyperplasia (BPH) (n = 7) and PCa patient samples (n = 14) (upper panel) and correlation analysis between METTL1 and WDR4 expression, AR and phospho-S6K (right panels). Statistical tests: ANOVA test (B, C), and log-rank Cox test (D). Data are represented as mean ± standard deviation (SD). Student’s t-test and Spearman’s correlation test (E)
Supplier Page from Abcam for Anti-WDR4 antibody [EPR11052]