Fig 1: Protein levels of Sema3C are upregulated in glioblastoma. a Representative example of a Western blot showing Sema3C protein expression in glioma tumor samples of differnet maligancy grades. Astrocytomas of WHO grade I, II, III, and IV (glioblastoma) are indicated as AI, AII, AIII, and GBM, respectively. Positions of Sema3C (Sema3C), ß-actin (Actin) and kDa values of the protein size marker (one before lane 1 and the second between lanes 12 and 13) are indicated. b Box plots of relative expression measurements of Sema3C obtained by Western blot analysis of astrocytoma samples. The line inside each box represents the median, the plus symbol (+) inside the box represent the mean, and the lower and upper edges of the boxes represent the 25th and 75th percentiles, respectively, and upper and lower lines outside the boxes represent minimum and maximum values (error bars). The Kruskal-Wallis test shows that there are statistically significant differences of relative Sema3C expression when glioblastomas are compared to grade I, II and III astrocytomas (p = 0.004, p < 0.0001, and p < 0.0001, respectively)