Fig 1: IL13Rα2-derived peptide inhibits IL13Rα1 and IL13Rα2-mediated signalling. a Cell lines were lysed and analysed by western blot to detect IL13Rα1 expression. RhoGDI was used as loading control. b IL13Rα1-positive cells were subjected to flow cytometry to detect IL13Rα1 expression on the cell surface. c HT-29 colon cancer and glioblastoma U251 cells were transfected with IL13Rα1 and IL13Rα2 siRNAs or control. At 48 h, transfected cells were lysed and subjected to western blot to confirm the silencing of expression. RhoGDI was used as loading control. d The same transfectants were used for IL-13 triggered invasion in the presence of Rα1 or D1 peptides. IL-13-mediated cell invasion (**p < 0.01; ***p < 0.001) was inhibited after IL13Rα2 silencing (§§p < 0.01; §§§p < 0.001) or by the addition of D1 peptide (◊◊p < 0.01; ◊◊◊p < 0.001). e Cells treated as in (d) were analysed by western blot to detect Src, phospho-Src, STAT6 and phospho-STAT6. Total Src and STAT6 were used as loading controls