Anti-human IFN-γ mAb (1-D1K), unconjugated 3420-3-1000 from Mabtech AB

Product Specs
ItemAnti-human IFN-γ mAb (1-D1K), unconjugated
CompanyMabtech AB
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3420-3-1000
IsotypeIgG1
Reactivityhuman
Clone1-D1K
ClonalityMonoclonal
HostMouse
Conjugate/Tagunconjugated
Quantity1 mg
ApplicationsELISpot,FluoroSpot,ELISA,Immunocytochemistry
Formatunconjugated
NCBI Gene AliasesIFG, IFI, IMD69
TargetIFN-γ
NCBI Full Gene Nameinterferon gamma
Concentration1 mg/ml
Puritypurified
Components1-D1K, purified, supplied at 1 mg/ml in PBS with 0.02% sodium azide

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) Exploring the immunogenicity of non-canonical HLA-I tumor ligands identified through proteogenomicsClinical Cancer ResearchJune 13, 2023Maria Lozano-Rabella, Andrea Garcia-Garijo, Jara Palomero, Anna Yuste-Estevanez, Florian Erhard, Roc Farriol-Duran, Juan Martín-Liberal, Maria Ochoa-de-Olza, Ignacio Matos, Jared J. Gartner, et al.(RRID:SCR_019597). In parallel, IFNy secretion was detected using IFNγ capture (MABTECH, catalog no. 3420–3-1000, RRID:AB_907282) and detection antibodies (MABTECH, catalog no. 3420–6-1000, RRID:AB_907272), and assessed

(2) Protocol to detect antigen-specific nasal-resident T cells in humansSTAR ProtocolsMarch 1, 2023Joey Ming Er Lim, Anthony T. Tan, Antonio BertolettiRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-human IFN-γ coating antibody (for ELISpot) dil. 1:200,Mabtech,Cat# 3420-3-1000; RRID: AB_907282 Anti-human IFN-γ biotin (for ELISpot) dil. 1:2000,Mabtech,Cat# 3420-6-1000; RRID:

(3) Ad26.COV2.S priming provided a solid immunological base for mRNA-based COVID-19 booster vaccinationiScienceJanuary 20, 2023Geers D, Sablerolles RSG, van Baarle D, Kootstra NA, Rietdijk WJR, Schmitz KS, Gommers L, Bogers S, Nieuwkoop NJ, van Dijk LLA, et al.HRP,Dako,Cat#P021402-2 Goat anti-rabbit IgG HRP,Dako,Cat#P044801-2 Anti-human IFN-γ clone 1-D1K,Mabtech,Cat#3420-3-1000; RRID:AB_907282 Anti-human biotinylated IFN-γ clone 7-B6-1,Mabtech,Cat#3420-6-1000; RRID:AB_907272 ,, Bacterial

(4) Attenuated humoral responses in HIV after SARS-CoV-2 vaccination linked to B cell defects and altered immune profilesiScienceJanuary 20, 2023Touizer E, Alrubayyi A, Ford R, Hussain N, Gerber PP, Shum HL, Rees-Spear C, Muir L, Gea-Mallorquí E, Kopycinski J, et al.421™ Streptavidin,BioLegend,Cat # 405226 "anti-human IFN-gamma mAb 1-D1K, purified",Mabtech,Cat # 3420-3-1000; RRID:AB_907282 "Anti-human IFN-γ mAb (7-B6-1), biotin",Mabtech,Cat # 3420-6-250; RRID:AB_907273 PE-Streptavidin,Agilent,Cat

(5) The making of multivalent gamma delta TCR anti-CD3 bispecific T cell engagersFrontiers in ImmunologyJanuary 5, 2023Eline van Diest, Mara J. T. Nicolasen, Lovro Kramer, Jiali Zheng, Patricia Hernández-López, Dennis X. Beringer, Jürgen Kuballnitrocellulose-bottomed 96-well plates (Millipore, cat no. MSIPN4550) precoated with α-IFNγ antibody (Mabtech, 3420-3-1000, clone 1-D1K 1:200). After 16 hours, the plates were washed with PBS and incubated with mAb7-B6-1 (II;

(6) A comparative characterization of SARS-CoV-2-specific T cells induced by mRNA or inactive virus COVID-19 vaccinesCell Reports MedicineNovember 15, 2022Joey Ming Er Lim, Shou Kit Hang, Smrithi Hariharaputran, Adeline Chia, Nicole Tan, Eng Sing Lee, Edwin Chng, Poh Lian Lim, Barnaby E. Young, David Chien Lye, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-human IFN-γ coating antibody,Mabtech,Cat# 3420-3-1000; RRID: AB_907282 Anti-human IFN-γ biotin,Mabtech,Cat# 3420-6-1000; RRID: AB_907272 Brilliant Violet

(7) Attenuated humoral responses in HIV infection after SARS-CoV-2 vaccination are linked to global B cell defects and cellular immune profilesbioRxivNovember 11, 2022Emma Touizer, Aljawharah Alrubbayi, Rosemarie Ford, Noshin Hussain, PehuÃ©n Pereyra Gerber, Hiu-Long Shum, Chloe Rees-Spear, Luke Muir, Ester Gea-MallorquÃ, Jakub Kopycinski, et al.(PHA-L),Sigma-aldrich,Cat # 11-249-738-001 "anti-human IFN-gamma mAb 1-D1K, purified",Mabtech,Cat # 3420-3-1000 MSCRN-IP DURA 0.45UM CLEAR 50/PK,Millipore,Cat # MAIPN4550 "mmPACT® AMEC Red Substrate, Peroxidase",2B

(8) CD4 T cell epitope abundance in ferritin core potentiates responses to hemagglutinin nanoparticle vaccinesNPJ vaccinesOctober 26, 2022Nelson SA, Richards KA, Glover MA, Chaves FA, Crank MC, Graham BS, Kanekiyo M, Sant AJ.with 10 μg/mL purified anti-human IL-2 (MT2A91/2C95, MabTech #3445-3-250) or IFNγ (1-D1K, MabTech #3420-3-1000) in PBS overnight at 4 °C. Prior to plating, wells were washed with media to remove unbound antibody,

(9) A phenotypic signature that identifies neoantigen-reactive T cells in fresh human lung cancersCancer CellMay 1, 2022Ken-ichi Hanada, Chihao Zhao, Raul Gil-Hoyos, Jared J. Gartner, Christopher Chow-Parmer, Frank J. Lowery, Sri Krishna, Todd D. Prickett, Scott Kivitz, Maria R. Parkhurst, et al.ELISPOT capture antibody,Mabtech,Cat# 3420-6-1000 Human IFNγ ELISPOT biotinylated antibody,Mabtech,Cat# 3420-3-1000 Anti-human CD3 antibody (OKT3),Invitrogen,Cat# 13-0037-85 Biological Samples,, Patient tumor and

(10) A Costimulatory CAR Improves TCR-based Cancer ImmunotherapyCancer immunology researchApril 1, 2022Omer B, Cardenas MG, Pfeiffer T, Daum R, Huynh M, Sharma S, Nouraee N, Xie C, Tat C, Perconti S, et al.were plated in triplicate in a 96-well plate precoated overnight with immobilized anti-IFNγ (MabTech, #3420-3-1000) at 10 μL/mL as described previously (24). T2 cells pulsed with 1 μL of survivin peptide (0.1 mol/L)

(11) Dominant CD8+ T Cell Nucleocapsid Targeting in SARS-CoV-2 Infection and Broad Spike Targeting From VaccinationFrontiers in ImmunologyFebruary 22, 2022Ellie Taus et al.ELISpot assay. Cells were added to a 96-well filter plate that had been pre-coated with an anti-IFN-γ antibody (#3420-3-1000, Mabtech, Nacka Strand, Sweden) with the addition of a peptide pool, medium alone (three wells), or

(12) Differential immunogenicity of homologous versus heterologous boost in Ad26.COV2.S vaccine recipientsMed (New York, N.Y.)February 11, 2022Khoo NKH, Lim JME, Gill US, de Alwis R, Tan N, Toh JZN, Abbott JE, Usai C, Ooi EE, Low JGH, et al.IgM Antibody,Biolegend,Cat# 314540; RRID: AB_2687215 Anti-human IFN-g coating antibody,Mabtech,Cat# 3420-3-1000; RRID: AB_907282 Anti-human IFN-g biotin,Mabtech,Cat# 3420-6-1000; RRID: AB_907272 "Streptavidin Protein,

(13) Discrete SARS-CoV-2 antibody titers track with functional humoral stabilityNature CommunicationsDecember 1, 2021Yannic C. Bartsch et al.plates (Millipore, MA, USA) were coated with anti-human IFNγ antibody (clone: 1-DK1, Mabtech Inc, #3420-3-1000, conc.: 2 μg/ml) overnight. Expanded and overnight rested PBMC samples (see above) were counted and

(14) Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onsetMed (New York, N.Y.)June 11, 2021Kalimuddin S, Tham CYL, Qui M, de Alwis R, Sim JXY, Lim JME, Tan HC, Syenina A, Zhang SL, Le Bert N, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-human IFN-γ coating antibody,Mabtech,Cat# 3420-3-1000; RRID:AB_907282 Anti-human IFN-γ biotin,Mabtech,Cat# 3420-6-1000; RRID:AB_907272 Anti-human CD3 BV605,Biolegend,Cat#

(15) Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patientsCell reportsFebruary 9, 2021Tan AT, Linster M, Tan CW, Le Bert N, Chia WN, Kunasegaran K, Zhuang Y, Tham CYL, Chia A, Smith GJD, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, anti-human IFN-γ coating antibody,Mabtech,Cat# 3420-3-1000; RRID:AB_907282 anti-human IFN-γ biotin,Mabtech,Cat# 3420-6-1000; RRID:AB_907272 anti-human CD3 PerCp

(16) Dynamics of CD4 T Cell and Antibody Responses in COVID-19 Patients With Different Disease SeverityFrontiers in MedicineNovember 11, 2020Maximilian Koblischke et al.(12, 13). Briefly, plates (MSIPS4W10, Merck-Millipore) were coated with 1.5 μg anti-IFN-γ antibody (3420-3-1000, Mabtech) per well. For blocking, PBS/5% BSA (11930, Serva) was used. The CD8-depleted PBMCs of COVID-19

(17) Sublethal Radiation Affects Antigen Processing and Presentation Genes to Enhance Immunogenicity of Cancer CellsInternational journal of molecular sciencesApril 7, 2020Punnanitinont A, Kannisto ED, Matsuzaki J, Odunsi K, Yendamuri S, Singh AK, Patnaik SK.Invitrogen®, Carlsbad, CA, USA), used at 1:100 dilution; IFNγ: mouse IgG1 mAb clone 1-D1K (product 3420-3-1000, Mabtech®), used at 15 µg/mL, and biotinymated mouse IgG1 mAb clone 7-B6-1 (product 3420-6-250, Mabtech®),

(18) Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humansNPJ vaccinesJanuary 1, 2020Richards KA, Moritzky S, Shannon I, Fitzgerald T, Yang H, Branche A, Topham DJ, Treanor JJ, Nayak J, Sant AJ.peptide pools on plates coated with anti-human interferon (IFN-γ) (clone 1-D1K, MabTech, catalogue number 3420-3-1000, 10μg/ml) for 36 h, washed and incubated with biotinylated anti-human IFN-γ (clone 7-B6-1, MabTech,

(19) Immunologic Recognition of a Shared p53 Mutated Neoantigen in a Patient with Metastatic Colorectal CancerCancer immunology researchApril 1, 2019Lo W, Parkhurst M, Robbins PF, Tran E, Lu YC, Jia L, Gartner JJ, Pasetto A, Deniger D, Malekzadeh P, et al.were washed 3 × with 200 μL PBS. IFNy capture antibody (1-D1K, stock 1 mg/mL, Mabtech, Inc.; cat no. 3420-3-1000) was diluted to 10 μg/mL (1:100) in PBS and plated at 50 μL/well. Plates were covered with plastic wrap

(20) Cytokines concentrations in serum samples from allergic children-Multiple analysis to define biomarkers for better diagnosis of allergic inflammatory processImmunobiologyNovember 1, 2018Kordulewska NK, Cieślińska A, Fiedorowicz E, Jarmołowska B, Piskorz-Ogórek K, Kostyra E.

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Anti-human IFN-gamma mAb (1-D1K), unconjugated

Anti-human IFN-γ mAb (1-D1K), unconjugated from Mabtech AB

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Mabtech AB

Citations (44)

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Anti-human IFN-γ mAb (1-D1K), unconjugated from Mabtech AB

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