Anti-human IFN-γ mAb (1-D1K), unconjugated 3420-3-250 from Mabtech AB

Product Specs
ItemAnti-human IFN-γ mAb (1-D1K), unconjugated
CompanyMabtech AB
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3420-3-250
Conjugate/Tagunconjugated
Clone1-D1K
Quantity250 µg
HostMouse
Reactivityhuman
ApplicationsELISpot,FluoroSpot,ELISA,Immunocytochemistry
Formatunconjugated
ClonalityMonoclonal
IsotypeIgG1
NCBI Gene AliasesIFG, IFI, IMD69
TargetIFN-γ
NCBI Full Gene Nameinterferon gamma
Concentration1 mg/ml
Puritypurified
Components1-D1K, purified, supplied at 1 mg/ml in PBS with 0.02% sodium azide

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) A H3K27M-targeted vaccine in adults with diffuse midline gliomaNature MedicineOctober 1, 2023Niklas Grassl, Isabel Poschke, Katharina Lindner, Lukas Bunse, Iris Mildenberger, Tamara Boschert, Kristine Jähne, Edward W. Green, Ingrid Hülsmeyer, Simone Jünger, et al.(Millipore, Merck, MSIPS4W10) were coated overnight at 4 °C with anti-human IFN-γ (1-D1K, Mabtech, 3420-3-250) and blocked with X-Vivo20 (Lonza) containing 1% BSA. PBMCs were thawed, rested in X-Vivo20 medium for

(2) A systems immunology study comparing innate and adaptive immune responses in adults to COVID-19 mRNA and adenovirus vectored vaccinesCell Reports MedicineMarch 21, 2023Feargal J. Ryan, Todd S. Norton, Conor McCafferty, Stephen J. Blake, Natalie E. Stevens, Jane James, Georgina L. Eden, Yee C. Tee, Saoirse C. Benson, Makutiro G. Masavuli, et al.RRID:AB_2869673 HLA-DR APC-H7,BD Biosciences,Cat#561358; RRID:AB_10611876 IFN-γ 1D1K purified,MabTech,Cat#3420-3-250; RRID:AB_907283 IFN-γ 7-B6-1 biotin,MabTech,Cat#3420-6-250; RRID:AB_907273 Siglec-8 PE-dazzle594,Biolegend,Cat#347109;

(3) Safety and immunogenicity of a thermostable ID93 + GLA-SE tuberculosis vaccine candidate in healthy adultsNature CommunicationsMarch 6, 2023Zachary K. Sagawa, Cristina Goman, Aude Frevol, Azra Blazevic, Janice Tennant, Bridget Fisher, Tracey Day, Stephen Jackson, Franck Lemiale, Leon Toussaint, et al.with 35% alcohol, coating plates with capture antibodies to either IFN-γ or IL-10 (Clone 1-D1K, Cat# 3420-3-250, or Clone 9D7, Cat# 3430-3-250, respectively, MabTech) at 100-fold dilution, and incubated overnight

(4) Impacts of delta and omicron variants on inactivated SARS‐CoV‐2 vaccine‐induced T cell responses in patients with autoimmune diseases and healthy controlsClinical and Translational MedicineJanuary 13, 2023Shuyi Wang, Jin Li, Shuang Wang, Yujin Ye, Mengyuan Li, Yihao Liu, Binfeng Chen, Yimei Lai, Liubing Li, Lili Zhuang, et al.listed in Table S5. For precoating, plates were incubated with anti‐human IFNγ antibody (MabTech, Cat#3420‐3‐250) in 100 μl PBS at a concentration of 15 μg/ml at 4°C overnight. The precoated plates were then washed

(5) WT1 Trio Peptide-Based Cancer Vaccine for Rare Cancers Expressing Shared Target WT1Cancers (Basel)January 6, 2023Yusuke Oji, Naoki Kagawa, Hideyuki Arita, Norifumi Naka, Ken-ichiro Hamada, Hidetatsu Outani, Yasushi Shintani, Yoshito Takeda, Eiichi Morii, Kenzo Shimazu, et al.interferon-gamma (IFN-γ) monoclonal Ab (anti-human IFN-γ mAb 1-D1K; MABTECH, Cincinnati, OH, USA, #3420-3-250, final concentration: 15 μg/mL in phosphate-buffered saline [PBS]), anti-human tumor necrosis factor-alpha

(6) Durable spike-specific T cell responses after different COVID-19 vaccination regimens are not further enhanced by booster vaccinationScience ImmunologyDecember 23, 2022Yacine Maringer, Annika Nelde, Sarah M. Schroeder, Juliane Schuhmacher, Sebastian Hörber, Andreas Peter, Julia Karbach, Elke Jäger, Juliane S. Walz96-well ELISpot plates coated with anti–IFN-γ antibody (2 μg/ml; clone 1-D1K, MabTech, catalog no. 3420-3-250, RRID: AB_907283) with peptide pools (0.01 nmol per peptide per milliliter). Phytohemagglutinin (Sigma-Aldrich)

(7) Cytotoxic T lymphocytes targeting a conserved SARS-CoV-2 spike epitope are efficient serial killersBioTechniquesApril 1, 2022Mohsen Fathi, Lindsey Charley, Laurence JN Cooper, Navin Varadarajan, Daniel D Meyer(Thermo Fisher Scientific, MA, USA, Cat #11033) with 40 μg/ml anti-human IFN-γ mAb 1-D1K (MABTECH, Stockholm, Sweden, Cat #3420-3-250) for 30 min, followed by two washes. AF647-Annexin V (Thermo Fisher Scientific, Cat #A23204) was used

(8) Robust induction of B cell and T cell responses by a third dose of inactivated SARS-CoV-2 vaccineCell discoveryFebruary 1, 2022Liu Y, Zeng Q, Deng C, Li M, Li L, Liu D, Liu M, Ruan X, Mei J, Mo R, et al.were performed as described previously27. Briefly, 100 μL of the coating antibody (15 μg/mL, MabTech, #3420-3-250) was added to the well of ELISpot plate (MabTech) and incubated overnight at 4 °C. The peptide pool

(9) WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccineOncology LettersJanuary 3, 2022Shouq Alzaaqi et al.IFN-γ monoclonal antibody (anti-human IFN-γ mAb 1-D1K; final concentration, 15 µg/ml in PBS; cat. no. 3420-3-250; Mabtech AB) and anti-human IL-10 monoclonal antibody (anti-human IL-10 mAb; final concentration, 15

(10) Functional analysis of peripheral and intratumoral neoantigen-specific TCRs identified in a patient with melanomaJournal for ImmunoTherapy of CancerSeptember 12, 2021Eva Bräunlein et al.release by ELISpot assay by coating ELISpot plates (MAHAS4510) with IFN-γ capture antibody (MABTECH cat# 3420-3-250) followed by application of IFN-γ-detection antibody (MABTECH cat# 3420-6-250) and visualization by

(11) Preexisting and Post–COVID-19 Immune Responses to SARS-CoV-2 in Patients with CancerCancer DiscoveryAugust 1, 2021Tatjana Bilich et al.96-well ELISPOT plates coated with anti-IFN-g Page 9/22 antibody (clone 1-D1K, 2 mg/mL, MabTech, Cat# 3420-3-250, RRID: AB_907283). PHA (Sigma-Aldrich) served as positive control. An irrelevant HLA-matched control

(12) Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targetingNature communicationsJuly 16, 2021Bear AS, Blanchard T, Cesare J, Ford MJ, Richman LP, Xu C, Baroja ML, McCuaig S, Costeas C, Gabunia K, et al.analyzed using FlowJo software (version 10.0.7). ELISPOT assays utilized anti-human IFN-γ 1-D1K (Mabtech, 3420-3-250, 1:100), and anti-human IFN-γ 7-B6-1—Biotin (Mabtech, 3420-6-1000, 1:1000). For rapid expansion of sorted

(13) T cell and antibody kinetics delineate SARS-CoV-2 peptides mediating long-term immune responses in COVID-19 convalescent individualsScience translational medicineApril 21, 2021Bilich T, Nelde A, Heitmann JS, Maringer Y, Roerden M, Bauer J, Rieth J, Wacker M, Peter A, Hörber S, et al.The 96-well plates were coated with anti–IFN- antibody (2 g/ml; clone 1-D1K, MabTech, catalog no. 3420-3-250, RRID: AB_907283). After a 24-hour incubation, spots were revealed with anti–IFN- biotinylated detection

(14) Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19CellOctober 1, 2020Sekine T, Perez-Potti A, Rivera-Ballesteros O, Strålin K, Gorin JB, Olsson A, Llewellyn-Lacey S, Kamal H, Bogdanovic G, Muschiol S, et al.

(15) Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T CellsCell metabolismSeptember 1, 2020Hamaidi I, Zhang L, Kim N, Wang MH, Iclozan C, Fang B, Liu M, Koomen JM, Berglund AE, Yoder SJ, et al.antibody,BioXCell,"Cat#BE0090, Clone: LTF-2; RRID: AB_1107780" Monoclonal Anti-Human IFN-γ antibody,"Mabtech, Inc.","Cat#3420-3-250, Clone: 1-D1K; RRID: AB_907283" Biotinylated anti-IFN-γ antibody,"Mabtech, Inc.","Cat#3321-6-250, Clone:

(16) Rapid Induction and Maintenance of Virus-Specific CD8+ TEMRA and CD4+ TEM Cells Following Protective Vaccination Against Dengue Virus Challenge in HumansFrontiers in ImmunologyMarch 24, 2020Nancy Graham et al.(Immobilon-P; Millipore) were pre-coated overnight with 50 μL of anti-human IFN-γ mAb 1-D1K (1 mg/mL) (3420-3-250; Mabtech). The next day, after washing the plates three times with PBS, 2 × 105 PBMC from each donor

(17) Reader‑free ELISPOT assay for immuno‑monitoring in peptide‑based cancer vaccine immunotherapyBiomedical ReportsMarch 5, 2020Sae Hayashi et al.was incubated with capture antibodies, anti‑human interferon‑γ (IFN‑γ) monoclonal antibody (cat. no. 3420‑3‑250; Mabtech AB; final concentration, 15 µg/ml in PBS) and anti‑human tumor necrosis factor‑α (TNF‑α) monoclonal

(18) The Immune Response to Eastern Equine Encephalitis Virus Acquired Through Organ TransplantationFrontiers in microbiologyJanuary 1, 2020Raabe V, Lai L, Xu Y, Huerta C, Wang D, Pouch SM, Burke CW, Piper AE, Gardner CL, Glass PJ, et al.washed with de-ionized water and PBS, and coated with 10 μg/mL of anti-human IFN-γ antibody (Mabtech #3420-3-250). Plates were washed with PBS, blocked with R10 media, and peptide pools in stimulation media [R10 containing

(19) Identification of a CD8+ T-cell response to a predicted neoantigen in malignant mesotheliomaOncoimmunologyJanuary 1, 2020Sneddon S, Rive CM, Ma S, Dick IM, Allcock RJN, Brown SD, Holt RA, Watson M, Leary S, Lee YCG, et al.nitrocellulose microtiter plates were coated with 1 µg/mL anti-interferon-γ (INFγ) antibody (Mabtech, 3420-3-250) overnight at 4°C. Samples and controls were incubated in RPMI-1640 media with 10% fetal calf serum

(20) Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopesNature communicationsApril 23, 2019Ferdosi SR, Ewaisha R, Moghadam F, Krishna S, Park JG, Ebrahimkhani MR, Kiani S, Anderson KS.Billerica, MA, USA) were coated overnight with 5 μg/well of anti-IFN-γ capture antibody (clone D1K, # 3420-3-250, Mabtech, USA) diluted in sterile PBS. Frozen PBMCs were thawed rapidly and recombinant human IL-2 (20

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Anti-human IFN-gamma mAb (1-D1K), unconjugated

Anti-human IFN-γ mAb (1-D1K), unconjugated from Mabtech AB

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Mabtech AB

Citations (34)

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Anti-human IFN-γ mAb (1-D1K), unconjugated from Mabtech AB

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