Fig 1: Neuroligins (NLGNs) levels in the pFC of ENU2 mice. Protein levels were quantified by densitometry after western blot analysis for total NLGNs and for NLGN1, 2, and 3 family members. Values were normalized to GAPDH loading control and are represented as a box plot of their distribution (min/max e median). (A) NLGNs (WT n = 7, ENU2 n = 8, p = 0.2162); (B) NLGN1 (WT n = 18, ENU2 n = 13, p = 0.0142); (C) NLGN2 (WT n = 13, ENU2 n = 14, p = 0.0266); and (D) NLGN3 (WT n = 17, ENU2 n = 14, p = 0.5430). Statistical analysis compared ENU2 values versus WT (* p < 0.05). Representative images of western blot analysis are shown. Molecular masses are indicated on the blots in kDa.