Fig 1: PGC-1a expression in the kidneys of aged or diabetic C57BL/6J mice. (a) Kidney sections from 7-month-old and 27-month-old mice were examined. (b) Kidney sections from 20-week-old db/m or db/db mice were examined. (c) Diabetes was induced by the intraperitoneal injection of 50 mg/kg STZ for 5 days, and kidney sections were examined. (a–c) Paraffin-embedded kidney sections were subjected to immunofluorescence staining using an anti-PGC-1a antibody (1:100; ab54481; Abcam) and anti-rabbit Alexa Fluor 588 (1:1,000; A11036; Invitrogen). Nuclei were stained with DAPI (Hwang et al., 2012). Images were taken using a Zeiss ApoTome Axiovert 200 M microscope (Carl Zeiss Microscopy GmbH). Scale bar indicates 50 µm. Representative images are shown
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