Fig 1: Knockdown of hsa_circ_0004396 inhibited tumor growth in NSCLC in vivo. (A and B) The tumor volume and weight were measured. (C and D) hsa_circ_0004396 and miR-615-5p were detected by qRT-PCR. (E and F) The mRNA and protein levels of PAK1 were detected by qRT-PCR and Western blot. (G and H) The expression levels of Ki67 and PAK1 were examined by Immunohistochemical staining. *p < 0.05
Fig 2: Hsa_circ_0004396 boosted NSCLC cell malignant behavior and radiosensitivity, by regulating the miR-615-5p/PAK1 axis
Fig 3: Hsa_circ_0004396 regulated migration and invasion by miR-615-5p/PAK1 in vitro. (A–C) A549 and SPC-A1 cells were transfected with sh-NC, sh-hsa_circ_0004396#1, sh-hsa_circ_0004396#1 + miR-615-5p inhibitor, or sh-hsa_circ_0004396#1 + PAK1. (A and B) Transwell assay was aimed to identify cell migration (A) and invasion (B) in transfected A549 and SPC-A1 cells. (C) The levels of Bcl-2, Bax, and MMP-2 protein in transfected A549 and SPC-A1 cells were analyzed by Western blot assay. *p < 0.05
Fig 4: Hsa_circ_0004396 regulated NSCLC cell malignant behavior and radiosensitivity by miR-615-5p/PAK1 in vitro. (A and B) PAK1 in NSCLC cells transfected with pcDNA3.1 or PAK1 were detected by qRT-PCR and Western blot. (C-L) A549 and SPC-A1 cells were transfected with sh-NC, sh-hsa_circ_0004396#1, sh-hsa_circ_0004396#1 + miR-615-5p inhibitor, or sh-hsa_circ_0004396#1 + PAK1 respectively. (C and D) PAK1 in NSCLC cell lines was examined via qRT-PCR assay and Western blot. (E and F) CCK-8 was used to detect cell proliferation of A549 (E) and SPC-A1 cells (F) after transfection. (G) Colony formation assay was conducted to examine the cell viability after transfection. (H and I) Colony formation assay detects the cell survival fraction. (J–L) cell cycle progression and apoptosis in tumor cells were assessed using flow cytometry. *p < 0.05
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