Fig 1: Il19 knockout in host mice suppresses lung cancer bone metastasis.(A) Genotyping (top) and IL-19 secretion by bone marrow cells (bottom) of Il19 WT (+/+) or KO (–/–) mice. (B–E) Intracardiac injection of LLC with or without Il20rb overexpression into Il19 WT or KO mice for bone metastasis analysis. Whole-body BLI analysis of tumor burden (B), ex vivo BLI analysis of hind limbs (C), micro-CT analysis of hind limbs (D, arrows point to osteolytic areas), and immunofluorescent (IF) analysis of EdU+ tumor cells in bone (E). (F) Organoid formation of A549 cells (with or without IL20RB overexpression) after treatment with OC CM from WT or KO mice. Scale bars: 100 µm. P values were obtained by Mann-Whitney U test (B and C) and 2-tailed unpaired t test (D–F). Scale bar: 100 µm. Data are represented as mean ± SD. Box plots display values of minimum, first quartile, median, third quartile, and maximum.