Fig 1: Several differentiation statuses were identified based on the cellular diversity within APGs(A) Partition-based graph abstraction connectivity among clusters of PIT1 lineage cells in APGs based on velocity-inferred directionality.(B) Integrated UMAP plot of PIT1 lineage cells from APGs with cells colored by Monocle3-inferred pseudotime.(C) Gene expression trends in particular genes along the differentiation trajectory from Pro.PIT1 toward SOMATO and LACTOHigh.(D) Integrated UMAP plot and Slingshot developmental trajectories of integrated adult and fetal APGs with cells colored according to the corresponding cell type.(E) mIHC staining of GH, NTS, PIT1, PRL, ENPP1, and TSHB in sections from adult APGs. Tissues were counterstained with DAPI. Scale bars, 50 µm(F) mIHC staining of GH, IGFBP7, PRL, LRRC4C, PIT1, and TSHB in sections from adult APGs. Tissues were counterstained with DAPI. Scale bars, 50 µm. See also Figure S3.
Fig 2: Single-cell transcriptome profiling of three adult human APGs(A) Overview of the study design and workflow.(B) Integrated UMAP plot of all 6,589 cells from three APGs with clusters outlined by lineages and cells colored according to the corresponding cell types. LACTO, lactotrope; SOMATO, somatotrope; THYRO, thyrotrope; GONADO, gonadotrope.(C) Integrated UMAP plot of APGs with cells colored according to the expression of classical marker genes of pituitary (PRL, GH1, TSHB, FSHB, POMC, and SOX2).(D) Dot plot of novel marker genes in endocrine cell types of the APG. The color represents the scaled relative expression level of the marker genes in each cell type, and the size indicates the proportion of cells expressing the marker genes.(E) mIHC staining of S100B, SOX2, CLDN4, SOX9, and VIM in sections from an adult APG. Tissues were counterstained with DAPI. The arrowheads indicate S100BPos STEM (SOX2+ S100B+). The arrows indicate S100BNeg STEM (SOX2+ S100B–). Scale bars, 50 µm. See also Figures S1 and S2.
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