GFP Antibody AF4240 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGFP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF4240
ApplicationsWestern Blot, Simple Western, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
ReactivityMulti-Species
Quantity100 ug (also 25 ug)
TargetGFP
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant GFPuv, Ser2-Lys238, Accession # P42212
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects GFP in direct ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A cell-based assay for rapid assessment of ACE2 catalytic functionScientific ReportsAugust 29, 2023Warren M. Meyers, Ryan J. Hong, Wun Chey Sin, Christine S. Kim, Kurt HaasPA520045, ThermoFisher Scientific), ACE2 (1:35 dilution, AF933, R&D Systems), or GFP (1:10 dilution, AF4240, R&D Systems) using the default separation parameters and detected with rabbit or goat chemiluminescent

(2) Extending the dynamic range of biomarker quantification through molecular equalizationNature communicationsJuly 13, 2023Newman SS, Wilson BD, Mamerow D, Wollant BC, Nyein H, Rosenberg-Hasson Y, Maecker HT, Eisenstein M, Soh HT.

(3) Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's diseaseBrain, behavior, and immunityMarch 1, 2023Erickson MA, Logsdon AF, Rhea EM, Hansen KM, Holden SJ, Banks WA, Smith JL, German C, Farr SA, Morley JE, et al.overnight at 4 °C with the following antibodies: Green fluorescent protein (GFP, Goat, R&D Systems: AF4240, 1:100), Ionized calcium binding adaptor molecule 1 (Iba1, Rabbit, WAKO: 019–19741, 1:1,000), and Glial

(4) Proteomics uncover EPHA2 as a potential novel therapeutic target in colorectal cancer cell lines with acquired cetuximab resistanceJournal of Cancer Research and Clinical OncologyFebruary 1, 2023Lucien Torlot, Anna Jarzab, Johanna Albert, Ágnes Pók-Udvari, Arndt Stahler, Julian Walter Holch, Marco Gerlinger, Volker Heinemann, Frederick Klauschen, Thomas Kirchner, et al.anti-EPHA2 (α-EPHA2, AF3035, R&D Systems, directed against EPHA2's extracellular domain) or anti-GFP (α-GFP AF4240, R&D Systems) antibodies at 5 µg/ml. Cells were used in assays 24 h after stimulation. Migration assay Pre-treated

(5) Intestinal inflammation and increased intestinal permeability in Plasmodium chabaudi AS infected miceWellcome Open ResearchOctober 5, 2022Jason P Mooney, Sophia M DonVito, Rivka Lim, Marianne Keith, Lia Pickles, Eleanor A Maguire, Tara Wagner-Gamble, Thomas Oldfield, Ana Bermejo Pariente, Ajoke M Ehimiyien, et al.blocking with avidin and biotin for 15min (927301, Bio Legend). Slides were incubated with goat anti-GFP (AF4240, R&D Systems) diluted 1:500 in PBS/0.1%Tween+1%FBS) in a humified chamber at 4°C overnight. After 3

(6) ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathwayLife Science AllianceJuly 1, 2022Marta Wróbel et al.and -polyubiquitinylated conjugates (Cat. no. BML-PW8810) from Enzo Life Sciences; anti-GFP (Cat. no. AF4240) from R&D Systems; anti-GAPDH (Cat. no. sc-25778) and anti-lamin A/C (Cat. no. sc-7292) from Santa Cruz;

(7) MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted HeartsInternational journal of molecular sciencesJanuary 8, 2022Pittenger MF, Eghtesad S, Sanchez PG, Liu X, Wu Z, Chen L, Griffith BP.

(8) Neonatal diabetes mutations disrupt a chromatin pioneering function that activates the human insulin geneCell reportsApril 13, 2021Akerman I, Maestro MA, De Franco E, Grau V, Flanagan S, García-Hurtado J, Mittler G, Ravassard P, Piemonti L, Ellard S, et al.anti-Glucagon,MilliporeSigma,4031-01F rabbit anti glucagon,Dako,A0565 Somatostatin,Dako,A0566 GFP,R&D Systems,AF4240 Immunoflourescence secondary antibodies,Jackson immunoResearch,"Cy3, Cy5 and Alexa flour 488" ,, Biological

(9) Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric NeuroinflammationCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Dora D, Ferenczi S, Stavely R, Toth VE, Varga ZV, Kovacs T, Bodi I, Hotta R, Kovacs KJ, Goldstein AM, et al.Hu (Abcam, Cambridge, MA; #96474), CD45 (BioLegend, San Diego, CA; #30F11), anti-GFP (R&D Systems; #AF4240), CD11b (Bio-Rad, Hercules, CA; #M1/70.15), CSF1R (Invitrogen, Waltham, MA; #PA5-25974), F4/80(BM8)

(10) Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapauseNature communicationsOctober 30, 2020Fan R, Kim YS, Wu J, Chen R, Zeuschner D, Mildner K, Adachi K, Wu G, Galatidou S, Li J, et al.610154, 1:300), mouse anti-E-Cadherin (BD Biosciences, 610182, 1:300), goat anti-GFP (R&D systems, AF4240, 1:300), rat anti-Podocalyxin (R&D systems, MAB1556, 1:300), rabbit anti-Cleaved Caspase-3 (Cell Signalling

(11) Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical NeuronsFrontiers in Cellular NeuroscienceOctober 19, 2020Nadine F. Joseph, Eddie Grinman, Supriya Swarnkar, Sathyanarayanan V. PuthanveettilSynaptic Systems), anti-PSD-95 (1:1,000, MA1-045, Thermo Fisher Scientific) and anti-GFP (1:4,000, AF4240, Novus Biologicals) in the blocking solution. After overnight incubation, cells were washed thoroughly

(12) Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cellseLifeAugust 14, 2020Cendrowski J, Kaczmarek M, Mazur M, Kuzmicz-Kowalska K, Jastrzebski K, Brewinska-Olchowik M, Kominek A, Piwocka K, Miaczynska M.anti-SQSTM1/p62 (610833), anti-μ2 (611350) and anti-SEC31A (612350) from BD Biosciences; anti-GFP (AF4240) from R&D Systems; anti-SEC16A (A300-648A) from Bethyl Laboratories Inc; anti-SEC16A (HPA005684) from

(13) Smooth Muscle Cell Reprogramming in Aortic AneurysmsCell stem cellApril 2, 2020Chen PY, Qin L, Li G, Malagon-Lopez J, Wang Z, Bergaya S, Gujja S, Caulk AW, Murtada SI, Zhang X, et al.anti-CD105,R and D Systems,"Cat# AF1320, RRID:AB_354735" Goat polyclonal anti-GFP,R and D Systems,"Cat# AF4240, RRID:AB_884445" Rabbit monoclonal anti-PPAR gamma (clone c26H12),Cell Signaling,"Cat# 2435, RRID:AB_2166051" Rabbit

(14) High-resolution mapping reveals that microniches in the gastric glands control Helicobacter pylori colonization of the stomachPLoS biologyMay 1, 2019Fung C, Tan S, Nakajima M, Skoog EC, Camarillo-Guerrero LF, Klein JA, Lawley TD, Solnick JV, Fukami T, Amieva MR.rotation. Antibodies and tissue-labeling reagents Goat anti-GFP (R&D Systems, Minneapolis, MN, Catalog #AF4240; RRID: AB_884445), rabbit anti-RFP (Rockland, Limerick, PA, Catalog #600-401-379; RRID: AB_2209751),

(15) Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probeNature CommunicationsDecember 1, 2018Troy F. Langford, Beijing K. Huang, Joseph B. Lim, Sun Jin Moon, Hadley D. SikesCatalog # AF3489) (at a dilution of 1:800) or goat primary antibody for GFP (R&D Systems, Catalog # AF4240) at 4 °C overnight and then with donkey anti-goat IR 688 (Licor) (at a dilution of 1:10,000) for 1 h

(16) Agrin has a pathological role in the progression of oral cancerBritish journal of cancerJune 1, 2018Rivera C, Zandonadi FS, Sánchez-Romero C, Soares CD, Granato DC, González-Arriagada WA, Paes Leme AF.Ct-agrin and IP-control cells. Immunoprecipitation was performed at 4 °C with 2.5 μg of GFP antibody (#af4240, R&D Systems) in the presence of 30 µL of protein G-Sepharose 4 Fast Flow (GE Healthcare) for 2 h in

(17) Natural killer cells attenuate cytomegalovirus-induced hearing loss in micePLoS pathogensAugust 1, 2017Almishaal AA, Mathur PD, Hillas E, Chen L, Zhang A, Yang J, Wang Y, Yokoyama WM, Firpo MA, Park AH.prior to application of primary antibodies. Primary antibodies to GFP (goat polyclonal anti-GFP, cat# AF4240, R&D Systems, Minneapolis, MN, USA) and tdTomato (rabbit polyclonal anti- RFP, cat#600-401-379, Rockland Antibodies,

(18) Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4PloS oneJanuary 1, 2017Volk-Draper LD, Hall KL, Wilber AC, Ran S.

(19) Efficient Generation of Cardiac Purkinje Cells from ESCs by Activating cAMP SignalingStem cell reportsJune 9, 2015Tsai SY, Maass K, Lu J, Fishman GI, Chen S, Evans T.incubated with antibodies against HCN4 (1:500, mouse; Abcam, ab85023), GFP (1:2,000, goat; R&D system, AF4240), TNNT2 (1:500, mouse, Thermo Scientific, MA5-12960), αMHC (1: 500, mouse, Abcam, ab15), Connexin43

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GFP Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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GFP Antibody from R&D Systems, a Bio-Techne Brand

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