Anti-Neutrophil antibody [7/4] ab53457 from Abcam

Product Specs
ItemAnti-Neutrophil antibody [7/4]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab53457
Antibody TypePrimary
IsotypeIgG2a
Quantity100 UG
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Frozen sections), Western blot
ClonalityMonoclonal
HostRat
Conjugate/TagUnconjugated
Clone43928
FormatPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Concentration1 mg/ml
Gene NameLy6b
ReactivityMouse
TargetNeutrophil

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Murine brain tumor microenvironment immunophenotyping using mass cytometrySTAR ProtocolsJune 17, 2022Brandon L. McClellan, Mahmoud S. Alghamri, Rohit Thalla, Pedro R. Lowenstein, Maria G. Castro153102 CD48a,147Sm,HM48-1,Biolegend,Cat# 103433 CD16/32a,148Nd,93,Biolegend,Cat# 101335 Ly-6Ba,149Sm,7/4,Abcam,Cat# ab53457 CD103a,151Eu,QA17A24,Biolegend,Cat# 121402 CXCR2a,155Gd,SA044G4,Biolegend,Cat# 149302 Ly-6A/E (Sca-1)a,158Gd,D7,Biolegend,Cat#

(2) The neutrophil enzyme myeloperoxidase modulates neuronal response in a model of subarachnoid hemorrhage by venous injuryStrokeOctober 1, 2021Aminata P. Coulibaly et al.GFAP and Vimentin. For meninges, whole mounts of the meninges were stained for neutrophils (NT 7/4; AB53457, Abcam, Cambridge, MA, US). All images were obtained on the Olympus FV1200 confocal microscope with

(3) The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodiesCell reportsApril 6, 2021Earnest JT, Holmes AC, Basore K, Mack M, Fremont DH, Diamond MS.L34965 PerCP-Cy5.5 anti-CD11b,BioLegend,Cat # 101207; RRID: AB_312790 FITC anti-Ly6B,Abcam,Cat # ab53457; clone: 7/4 PE-Cy7 anti-Ly6G,BioLegend,Cat # 115511; RRID: AB_313646 Pacific Blue anti-Ly6C,BioLegend,Cat

(4) Cannabinoid receptor type 1 antagonist inhibits progression of obesity‐associated nonalcoholic steatohepatitis in a mouse model by remodulating immune system disturbancesImmunity, Inflammation and DiseaseDecember 1, 2020Chin‐Chang Chen, Zi‐Yu Chang, Fuu‐Jen Tsai, Shih‐Yin Chen(dilution rate, 1:80; cat: ab133357; Abcam, Cambridge, UK), anti‐Neutrophil (dilution rate, 1:80; cat: ab53457; Abcam), and anti‐CB1 (dilution rate, 1:80; cat: ADI‐905‐708; Enzo Life Science Inc, PA). Subsequently,

(5) Neutralization of Lipocalin-2 Diminishes Stroke-Reperfusion InjuryInternational journal of molecular sciencesAugust 29, 2020Wang G, Weng YC, Chiang IC, Huang YT, Liao YC, Chen YC, Kao CY, Liu YL, Lee TH, Chou WH.Glostrup, Denmark; 1:1000 dilution in IHC antibody diluent buffer), and rat anti-Ly-6B.2 clone 7/4 (Cat# ab53457, Abcam; 1:200 dilution in IHC antibody diluent buffer). After washing, the sections were stained with

(6) Signaling pathways predisposing to chronic kidney disease progressionJCI insightMay 7, 2020Zaidan M, Burtin M, Zhang JD, Blanc T, Barre P, Garbay S, Nguyen C, Vasseur F, Yammine L, Germano S, et al.(1:100, ab16667, Abcam, Inc.), CD3 (1:100; ab16669; Abcam, Inc.), F4/80 (1:100; MCA497; AbD Serotec), Ly6B (1:100, ab53457, Abcam, Inc.), phospho–histone H3 (Ser10) (1:100; 9716, Cell Signaling Technology), p-H2A.X (Ser139)

(7) Tubular STAT3 Limits Renal Inflammation in Autosomal Dominant Polycystic Kidney DiseaseJournal of the American Society of NephrologyMay 1, 2020Amandine Viau, Maroua Baaziz, Amandine Aka, Manal Mazloum, Clément Nguyen, E. Wolfgang Kuehn, Fabiola Terzi, Frank BienaiméCell Signaling Technology), F4/80 (1:100, clone Cl:A31, MCA497R; Bio-Rad), CD3 (1:100, ab16669; Abcam), LY6B (1:100, ab53457; Abcam), CXCL10 (1:100, ab9807; Abcam), and KI67 (1:100, ab15580; Abcam). Terminal Deoxynucleotidyl

(8) Majie cataplasm provides a shield against asthmatic punch from the neuroimmune systemHeliyonMay 1, 2020Ji W, Yu X, Gao Y, Ren B, Zhang S, Wang Q, Wang X.used: mouse monoclonal anti-CGRP antibody (ab81887, 1:200) and Rat Monoclonal anti-Neutrophil antibody (ab53457, 1:80) were from Abcam (Cambridge, UK). Rabbit polyclonal anti-CD3 (17617-1-AP, 1:170) was from Proteintech

(9) Neutrophil extracellular traps exacerbate neurological deficits after traumatic brain injuryScience advancesMay 1, 2020Vaibhav K, Braun M, Alverson K, Khodadadi H, Kutiyanawalla A, Ward A, Banerjee C, Sparks T, Malik A, Rashid MH, et al.Tween-20 in PBS and then incubated with primary antibodies [rat anti-mouse neutrophil 7/4 (Abcam no. ab53457) 1:200 or goat anti-mouse MPO (R&D System no. AF3667) 1:200] overnight at 4°C followed by incubation

(10) MicroRNA-146a-deficient mice develop immune complex glomerulonephritisScientific ReportsDecember 1, 2019Lucile Amrouche et al.1:400), monoclonal rabbit anti-mouse CD8 (Abcam, ab209775, 1:100), monoclonal rat anti-mouse Ly6b (Abcam, ab53457, 1:100) and monoclonal rat anti-mouse CD-68 (Abcam, ab53444, 1:100). Briefly, deparaffinized kidney

(11) The Mixed-Lineage Kinase Inhibitor URMC-099 Protects Hippocampal Synapses in Experimental Autoimmune EncephalomyelitiseneuroNovember 16, 2018Matthew J. Bellizzi, Jennetta W. Hammond, Herman Li, Mary A. Gantz Marker, Daniel F. Marker, Robert S. Freeman, Harris A. Gelbard(Abd Serotec, catalog #MCA1957, RRID:AB_322219; 1:1000), rat anti-Ly-6B (clone 7/4, Abcam, catalog #ab53457, RRID:AB_881409; 1:1000), or mouse anti-MAP2 (Sigma, catalog #M4403, RRID:AB_477193; 1:500). We washed

(12) Beneficial Role of Neutrophils Through Function of Lactoferrin After Intracerebral HemorrhageStrokeMay 1, 2018Xiurong Zhao et al.performed as described earlier.24 Rabbit anti-LTF (L3262; Sigma) and rat anti-mouse neutrophil antibody (ab53457; Abcam) were used to visualize LTF and PMNs. The nuclei were visualized with DAPI. For PMNs counting,

(13) Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhageNature communicationsSeptember 19, 2017Zhao X, Ting SM, Liu CH, Sun G, Kruzel M, Roy-O'Reilly M, Aronowski J.with tail blood smears on slides after immunofluorescent labeling with a rat anti-PMN antibody (ELANE, ab53457, Abcam). The digitized images (4 × 4 images under 20× lens) were acquired and stitched and the number

(14) Recovery of Corneal Sensitivity and Increase in Nerve Density and Wound Healing in Diabetic Mice After PEDF Plus DHA TreatmentDiabetesSeptember 1, 2017Jiucheng He, Thang Luong Pham, Azucena Kakazu, Haydee E.P. Bazanand Other Materials Rabbit monoclonal anti-PGP9.5 (EPR4118), rat monoclonal [7/4] anti-neutrophil (ab53457), and rabbit polyclonal anti– C-type mannose receptor 1 (CD206; ab64693) antibodies were purchased from

(15) MicroRNA-146a in Human and Experimental Ischemic AKI: CXCL8-Dependent Mechanism of ActionJournal of the American Society of NephrologyFebruary 1, 2017Lucile Amrouche et al.anti– mouse CD3 (1:100; ab16669; Abcam, Inc., Cambridge, MA), and monoclonal rat anti–mouse Ly6b (1:100; ab53457; Abcam, Inc.). Brieﬂy, deparafﬁnized kidney sections were boiled in citrate buffer for antigen retrieval.

(16) Understanding Lung Deposition of Alpha-1 Antitrypsin in Acute Experimental Mouse Lung Injury Model Using Fluorescence MicroscopyInternational Journal of Molecular ImagingDecember 6, 2016Mengmeng Wang, Yutian Zhan, Jianqing Chen, Haojing Rong, Shawn P. O’Neil, Brahma Ghosh, Vuong Nguyen, Jane Owens, Xianfeng Li, Denise M. O’Haratissue from the NE680 probe study using a rat anti-mouse neutrophil monoclonal antibody (clone 7/4, ab53457; Abcam, Cambridge, MA). IHC assays were performed as immunoperoxidase reactions on the Ventana Discovery

(17) Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord InjuryInternational Journal of Molecular SciencesAugust 23, 2016Zhijian Cheng, Wen Zhu, Kai Cao, Fei Wu, Jin Li, Guoyu Wang, Haopen Li, Ming Lu, Yi Ren, Xijing HeNumber,Dilution,Source GFAP,Ab53554,1:600,Abcam iNOS,610329,1:600,BD Biosciences Nestin,Ab24692,1:300,Abcam Neutrophil (7/4),AB53457,1:400,Abcam O4,MAB1326,1:100,R & D Systems Tuj1,04-1049,1:400,Millipore Primary antibodies used

(18) Leucine-rich repeat kinase 2 modulates neuroinflammation and neurotoxicity in models of human immunodeficiency virus 1-associated neurocognitive disordersThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 1, 2015Puccini JM, Marker DF, Fitzgerald T, Barbieri J, Kim CS, Miller-Rhodes P, Lu SM, Dewhurst S, Gelbard HA.mouse anti-microtubule-associated protein 2 (MAP2) at 1:500 (catalog #M4403; Sigma), rat anti-7/4 at 1:1000 (catalog #ab53457; Abcam), rabbit anti-synapsin-1 at 1:400 (catalog #5297; Cell Signaling Technology), guinea pig anti-VGluT

(19) Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancyThe Journal of biological chemistryJanuary 23, 2015Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce JS, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K.

(20) Interferon Regulatory Factor 7 Protects Against Vascular Smooth Muscle Cell Proliferation and Neointima FormationJournal of the American Heart Association: Cardiovascular and Cerebrovascular DiseaseOctober 10, 2014Ling Huang, Shu-Min Zhang, Peng Zhang, Xiao-Jing Zhang, Li-Hua Zhu, Ke Chen, Lu Gao, Yan Zhang, Xiang-Jie Kong, Song Tian, Xiao-Dong Zhang, Hongliang LiCambridge, UK), mouse anti‐PCNA (#2586; 1:100; Cell Signaling Technology, Beverly, MA), rat anti‐7/4 (ab53457; 1:50; Abcam, Cambridge, UK), rabbit anti‐CD3 (ab5690; 1:50; Abcam, Cambridge, UK), and rat anti‐Mac3

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Anti-Neutrophil antibody [7/4] from Abcam

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Anti-Neutrophil antibody [7/4] from Abcam

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