Anti-Biotin antibody ab53494 from Abcam

Product Specs
ItemAnti-Biotin antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab53494
Antibody TypePrimary
Quantity1 mg
ApplicationsCompetitive ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
HostRabbit
IsotypeIgG
ClonalityPolyclonal
Conjugate/TagUnconjugated
FormatAffinity purified with d-Biotin-agarose beads
Concentration1 mg/ml
ReactivitySpecies independent
TargetBiotin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in femalesRedox BiolDecember 6, 2023Varun Kumar, Neha Deshpande, Mohit Parekh, Raymond Wong, Shazia Ashraf, Muhammad Zahid, Hanna Hui, Annie Miall, Sylvie Kimpton, Marianne O. Price, et al.10 % normal goat and donkey serum (1:1) for 1 h at RT. The cells were incubated with anti-biotin (#ab53494, Abcam, USA) and Tom-20 (#sc-136211, Santa Cruz, USA) antibodies diluted in 5 % bovine serum albumin (BSA

(2) Single-cell signaling analysis reveals that Major Vault Protein facilitates RasG12C inhibitor resistancebioRxiv : the preprint server for biologyOctober 4, 2023Zhang JZ, Ong SE, Baker D, Maly DJ.anti-EGFR,Abcam,ab30 Rb anti-FGFR3,CST,4574S Gt anti-Biotin,Vector Labs,SP-3000-1 Rb anti-Biotin,Abcam,ab53494 Ms anti-Biotin,Abcam,ab201341 Rb anti-KRas,Millipore,OP24 Ms anti-HRas,Proteintec h,18295-1-AP Rb

(3) PerTurboID, a targeted in situ method reveals the impact of kinase deletion on its local protein environment in the cytoadhesion complex of malaria-causing parasiteseLifeSeptember 22, 2023Heledd Davies, Hugo Belda, Malgorzata Broncel, Jill Dalimot, Moritz Treeck4°C. Anti-biotin antibodies were added (equal mix of Bethyl Laboratories, no. 150-109A, and Abcam no. Ab53494), 30 µg of each antibody per 60 µL of bead slurry, made up to 2 mL with BioSITe buffer, and incubated

(4) Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neuronsSci AdvAugust 9, 2023Anqi Zhang, Kang Yong Loh, Chandan S. Kadur, Lukas Michalek, Jiayi Dou, Charu Ramakrishnan, Zhenan Bao, Karl Deisserothstained using the detergent-permeabilized staining protocol, with primary antibody against biotin (Abcam, ab53494) at 1:200 dilution, followed by Alexa Fluor 647 goat anti-rabbit secondary antibody, as described above. Scanning electron microscopy

(5) Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human CellsInternational Journal of Molecular SciencesMay 31, 2023Sébastien Meurant, Lorris Mauclet, Marc Dieu, Thierry Arnould, Sven Eyckerman, Patricia RenardNovus Biologicals, Abingdon, UK); mouse monoclonal CPT1A (ab128568, Abcam); rabbit polyclonal Biotin (ab53494, Abcam); rabbit polyclonal LARP4 (ab241489, Abcam); and mouse monoclonal α-tubulin (TUBA4A (7277)) (926-68070,

(6) Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cellsScientific ReportsApril 8, 2023Vojtěch Pavluch, Tomáš Špaček, Hana Engstová, Andrea Dlasková, Petr Ježek(IgM-based; Progen Biotechnik, Heidelberg, Germany, Cat# 61014; RRID:AB_2750935) or anti-biotin (Abcam, Cat# ab53494; RRID:AB_867860) were used. For Fig. 2h also rabbit anti-mtSSB (Sigma, St Louis, MO, USA) and rabbit

(7) High aspect ratio nanomaterial-induced macrophage polarization is mediated by changes in miRNA levelsFrontiers in ImmunologyJanuary 27, 2023Johanna Samulin Erdem, Táňa Závodná, Torunn K. Ervik, Øivind Skare, Tomáš Hron, Kristine H. Anmarkrud, Anna Kuśnierczyk, Julia Catalán, Dag G. Ellingsen, Jan Topinka, et al.immunogold labeling. Accordingly, the washed sections were incubated with a rabbit anti-biotin antibody (ab53494, Abcam, Cambridge, UK) at 1:300 dilution in 1% BSA-PBS for 30 min, followed by repeated washing in 0.1%

(8) KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cellsCommunications biologyDecember 5, 2022Zhang X, Zheng B, Zhao L, Shen J, Yang Z, Zhang Y, Fan R, Zhang M, Ma D, Zheng L, et al.DUO92101). After blocking, sections were incubated with mouse H3K4dime (ab6000, Abcam) and rabbit Biotin (ab53494, Abcam) antibodies overnight at 4 °C, followed by incubation with secondary antibodies conjugated with

(9) DDX17 helicase promotes resolution of R-loop-mediated transcription-replication conflicts in human cellsNucleic acids researchNovember 28, 2022Boleslavska B, Oravetzova A, Shukla K, Nascakova Z, Ibini ON, Hasanova Z, Andrs M, Kanagaraj R, Dobrovolna J, Janscak P.antibodies were used for western blotting (WB), immunofluorescence staining (IF) or slot blot: anti-biotin (ab53494, Abcam; WB, 1:500), anti-RNase H1 (in-house made, rabbit polyclonal; WB, 1:2500), anti-TFIIH (sc-293,

(10) TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switchMolecular cellNovember 3, 2022Yadav T, Zhang JM, Ouyang J, Leung W, Simoneau A, Zou L.,Millipore Sigma,Cat# MABE1095; RRID: AB_2861387 RAD52,Abcam,Cat# ab124971; RRID: AB_10971685 Biotin,Abcam,Cat# ab53494; RRID: AB_867860 GAPDH,Millipore,Cat# ABS16; RRID: AB_10806772 Ku80,NeoMarker,Cat# MS-285 P; RRID:

(11) Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse modelseLifeOctober 4, 2022Ramani K, Mavila N, Abeynayake A, Tomasi ML, Wang J, Matsuda M, Seki E.milk/TBS-Tween; PLA: (1:250) dilution in PLA buffer Antibody,Anti-Biotin antibody (rabbit polyclonal IgG),Abcam,ab53494,Western: (1:1000) in 5% milk/TBS-Tween-20 Antibody,Anti-GAPDH antibody (rabbit polyclonal IgG),Proteintech,10494-1-AP,Western:

(12) An optimized proximity ligation assay to detect telomere dysfunction induced foci in human and mouse cellsSTAR protocolsSeptember 16, 2022Wang Y, Ferrucci L, Seidman MM, Liu Y.AB_258625",Sigma-Aldrich,Cat#B7653 "Anti-Biotin antibody (Rabbit polyclonal, 1: 500)PRID: AB_867860",Abcam,Cat#ab53494 "Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (against Rabbit TRF2 and 53BP1, 1: 500)PRID: AB_2630356",Abcam,Cat#ab150077 "Goat Anti-Mouse IgG H&L (Alexa Fluor® 488

(13) Protocol to stimulate and delineate alternative lengthening of telomeres in human U2OS cellsSTAR ProtocolsSeptember 16, 2022Jia-Min Zhang, Lee Zouor RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-Biotin (1:1000),Abcam,Cat#ab53494; RRID: AB_867860 Anti-BLM (1:1000),Bethyl,Cat#A300-110A; RRID: AB_2064794 Alexa Fluor® 594 AffiniPure

(14) Dense Granule Protein GRA64 Interacts with Host Cell ESCRT Proteins during Toxoplasma gondii InfectionmBioAugust 30, 2022Mayoral J, Guevara RB, Rivera-Cuevas Y, Tu V, Tomita T, Romano JD, Gunther-Cummins L, Sidoli S, Coppens I, Carruthers VB, et al.dense granules with in-house mouse anti-GRA1 (MAb 92.10B; 1:1000), and biotin with anti-biotin (Abcam, ab53494, 1:1,000). Appropriate secondary antibodies conjugated to Alexa Fluorophores 488, 555, 594, and 633

(15) The roles of HMGB1 ‐produced DNA gaps in DNA protection and aging biomarker reversalFASEB BioAdvancesJune 1, 2022Sakawdaurn Yasom et al.(ab1162)(Abcam), anti‐SIRT1 mouse monoclonal antibody (ab110304)(Abcam), anti‐biotin rabbit polyclonal antibody (ab53494)(Abcam), and anti‐γH2A.X rabbit monoclonal antibody (9817 s)(Cell Signaling) antibodies were used, and

(16) Promoters of ASCL1- and NEUROD1-dependent genes are specific targets of lurbinectedin in SCLC cellsEMBO molecular medicineApril 7, 2022Costanzo F, Martínez Diez M, Santamaría Nuñez G, Díaz-Hernandéz JI, Genes Robles CM, Díez Pérez J, Compe E, Ricci R, Li TK, Coin F, et al.2016), Anti‐Phospho‐Serine 2 RNAPII (Abcam ab5095), γH2AX (Abcam ab2893), Anti‐Biotin antibody (Abcam ab53494), and Histone H3K27Ac antibody (pAb) (Active motif) overnight at 4°C. Antigen–antibody complexes were

(17) Lipid phosphate phosphatase 3 in smooth muscle cells regulates angiotensin II-induced abdominal aortic aneurysm formationScientific reportsApril 5, 2022Van Hoose PM, Yang L, Kraemer M, Ubele M, Morris AJ, Smyth SS.incubated with mouse H3K4dime (5 μg/mL, Millipore Sigma, clone CMA303) and rabbit Biotin (5 μg/mL, Abcam #ab53494) antibodies overnight at 4 °C, followed by incubated with secondary antibodies containing PLA probe

(18) Universal activity-based labeling method for ammonia- and alkane-oxidizing bacteriaThe ISME JournalApril 1, 2022Dimitra Sakoula, Garrett J. Smith, Jeroen Frank, Rob J. Mesman, Linnea F. M. Kop, Pieter Blom, Mike S. M. Jetten, Maartje A. H. J. van Kessel, Sebastian Lückerbiotinylated enzymes was performed according to Slot and Geuze (2007) using a rabbit anti-biotin antibody (ab53494, Abcam plc., Cambridge, UK, diluted 1:600 in 1% BSA-C) and 10 nm protein A gold (CMC-UMC Utrecht, the

(19) Immune microenvironment infiltration landscape and immune-related subtypes in prostate cancerFrontiers in immunologyJanuary 1, 2022Wu W, Wang X, Le W, Lu C, Li H, Zhu Y, Chen X, An W, Xu C, Wu Q, et al.h at room temperature. The cells were incubated with a primary antibody—anti-Biotin (Rabbit, Abcam, ab53494)—diluted in blocking solution for 2 h overnight at 4°C, washed three times with PBST, incubated with

(20) H3K4 di-methylation governs smooth muscle lineage identity and promotes vascular homeostasis by restraining plasticityDevelopmental CellOctober 1, 2021Mingjun Liu, Cristina Espinosa-Diez, Sidney Mahan, Mingyuan Du, Anh T. Nguyen, Scott Hahn, Raja Chakraborty, Adam C. Straub, Kathleen A. Martin, Gary K. Owens, et al.least 4 hours at 4°C. 7 μg of rabbit anti-HA antibody (Abcam, ab9110) and anti-Biotin antibody (Abcam, ab53494) was used to Immunoprecipitate HA-TET2 or Biotinylated H3K4me2 peptide respectively in presence of Dynabeads

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9/22/2015Extremely Strong Antibody Against Biotin Samyabrata BhaduriThis antibody detects trace amounts of biotinylated proteins with great ease at the recommended dilution.

Anti-Biotin antibody from Abcam

Abcam

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Anti-Biotin antibody from Abcam

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