Fig 1: Oct1 protein levels correlate with a stem cell phenotype in primary human malignancy.A. IF images are shown. Frozen malignant breast carcinoma sections (non-familial stage IIIA node-positive infiltrating ductal carcinoma, ER+PR+HER2-) were stained with mouse anti-Oct1 antibodies (Millipore MAB5434) and rabbit anti-ALDH1 antibodies (Abcam ab52492). Arrow indicates example double-positive cell. Asterisks show examples of an Oct1HI cell with low ALDH1 and ALDH1HI with low Oct1. Detail is shown in images below. B. Western blot depicting Oct1 levels in a panel of malignant epithelial cells isolated from pleural effusions (human breast carcinoma lung metastases). GAPDH is shown as a loading control. C. Examples of the CD24/44 profile from pleural effusions with highest and lowest Oct1 protein levels. D. Correlation of Oct1 protein and CD24LOCD44HI stem content in 15 individual patient samples (7 ER+PR+HER2-, 5 ER-PR-HER2-, 3 ER-PR-HER2+) collected from 14 different patients. One patient had tumor cells collected twice 22 months apart. Samples were placed into Oct1LO and Oct1HI categories based on Oct1 Western blot signal intensity relative to GAPDH staining. Cells were tested for percentage CD24LOCD44HI stem cell content and plotted. P-value was calculated using the two-tailed student T-test. E. The same analysis for Oct1 mRNA as measured by qRT-PCR.
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