Fig 1: Chromatids from micronuclei fail to activate the SAC and have impaired recruitment of kinetochore proteins. (A) Mitotic timing of cells from Fig. 1C. Only cells displaying very clear misalignment of the DNA derived from the micronuclei were analysed (MN+, n=17) and compared to untreated cells (MN-, n=50). Graph displays mean+s.d. (B) Experimental setup to stain for proteins on micronuclei-derived chromatids. ProTame prevented mitotic exit. Mps1i, MPS1 inhibitor (NMS-P715); CENP-Ei, CENP-E inhibitor (GSK923295). (C) Representative images of RPE-1 p53kd cells displaying reduced levels of Mad1 and Aurora B at micronuclei-derived chromatids. (D) Quantification of fluorescence intensity in cells from C. Data are displayed in box-and-whisker diagrams where the box represents the 25–75th percentiles, and the median is indicated. The whiskers show the range. n>6 cells/condition from two independent experiments. (E,F) Same as in C and D but cells were stained for CENP-A. n>12 cells/condition from two independent experiments. a.u., arbitrary units. Scale bars: 10 µm.