Fig 1: ANXA1 localisation to the plasma membrane is required for the patterning of LGN-NuMA-Dynein-Dynactin at the lateral cortex during metaphase.a Confocal images of representative MCF-10A cells treated with DMSO (Control) or 20 µM TG100-115 for 2 h, stained for ANXA1 (magenta) and S100A11 (green), and counterstained with DAPI (DNA, blue). b Average cortical and cytoplasmic fluorescence intensity profiles of ANXA1 and S100A11 from metaphase cells (Control: n = 30; TG100-115: n = 30). c Confocal images of representative MCF-10A cells treated with DMSO (Control) or 20 µM TG100-115 for 2 h, stained for LGN, NuMA or p150Glued (green), and counterstained with DAPI (DNA, magenta). d Percentage of cortical localisation of LGN, NuMA or p150Glued in Control and TG100-115-treated metaphase cells: LGN (Control: n = 39 cells; TG100-115: n = 37), NuMA (Control: n = 43 cells; TG100-115: n = 40), p150Glued (Control: n = 31 cells; TG100-115: n = 32). Two-sided t test, LGN (bilateral: ****P = 0.00005; unilateral: ***P = 0.0008; central: **P = 0.006; circumferential: **P = 0.01); NuMA (bilateral: ***P = 0.001; unilateral: *P = 0.044; central: **P = 0.005; circumferential: ****P = 0.00009; absent: P = 0.405); p150Glued (bilateral: ***P = 0.0002; unilateral: P = 0.728; absent: **P = 0.005). All data are presented as mean ± s.e.m. from 3 independent experiments. arb. units (arbitrary units). All scale bar, 10 µm. Source data are provided as a Source Data file.