Fig 1: Genetic disruption of PAD4, NE and ROS in BM improves ferritin-induced inflammation.a Experimental overview: Bone marrow (BM) from WT, Padi4-/-, Elane-/- and Cybb-/- mice in C57BL6 background were transplanted to WT recipients and allowed to reconstitute for 4 weeks following which ferritin was injected. b Quantification of cell-free DNA released by BMDNs (n = 3). c, d Western blotting analysis for NET markers citH3 and NE in the livers (WT > WT + PBS: n = 5, WT > WT + Ferritin: n = 6, Padi4-/- >WT + Ferritin: n = 6, Elane-/- >WT + Ferritin: n = 4, Cybb-/- >WT + Ferritin: n = 6). Representative Western blotting was shown. e–g Peripheral blood and liver neutrophil (e), changes of liver to body weight ratio (f) and serum cytokines, IL-6, MCP-1 and TNF-a (g) were evaluated in mice transplanted with Padi4-/-, Elane-/- and Cybb-/-BM (WT > WT + PBS: n = 5, WT > WT + Ferritin: n = 6, Padi4-/- >WT + Ferritin: n = 6, Elane-/- >WT + Ferritin: n = 4, Cybb-/- >WT + Ferritin: n = 6). h–j liver mRNA expression of Il1b, Il6 and Tnfa (h), H&E staining of liver inflammatory infiltration (i), and serum ALT levels (j) (WT > WT + PBS: n = 5, WT > WT + Ferritin: n = 6, Padi4-/- >WT + Ferritin: n = 6, Elane-/- >WT + Ferritin: n = 4, Cybb-/- >WT + Ferritin: n = 6). Scale bars, 50 µm. Data are presented as means ± SEM; ns not significant. One-way ANOVA (b, d, e–h, j). Source data are provided as a Source Data file.