Fig 2: Differential expression of O6-methylguanine DNA methyltransferase (MGMT) protein in mycosis fungoides (MF) and Sézary syndrome (SS) cells.Total cell lysates were subjected to Western blot analysis for MGMT expression. For comparison purposes, lysates from two glioblastoma cell lines, U251 (MGMT-negative) and T98G (MGMT-positive) were included. Actin was used as a loading control.

Fig 3: Molecular characterization of patient-derived GBM samples. (a) Representative H&E stain and immuno-histochemistry results of molecular markers showed the expression of GFAP, and Ki67 and IDH1 R132H were probed. (b) MGMT promoter methylation status was determined by measuring the percentage of methylated MGMT promoter in these five indicated CpG regions. Mean range of the regions <10%,10–26%, and ≧27% was considered as unmehtylated, intermediate methylated, and menthylated, respectively. (c) Detection of IFNγ in the patient's serum from pre-operative day 0 to day 14 post-surgery. Baseline values on day 0 were 13.25 pg per milliliter, and the increase in levels of IFNγ appeared to correspond with CWs implantation. After 3 days, IFNγ levels started to decline in a time-dependent manner, which correlate with the slow release of (Z)-BP.

Fig 4: Effects of MGMT expression on multidrug sensitivity of lung cancer cells. MGMT and Trps1 gene expressions were analyzed at mRNA (A) and protein (B) levels in MGMT stably overexpressed H446 cells. *P < 0.05 versus H446 and H446‐Le/GFP control groups; (C) the IC50 value of cisplatin in MGMT overexpressed H446 cells. (D) The IC50 value of 5‐FU in MGMT overexpressed H446 cells. *P < 0.05 versus H446 and H446‐Le/GFP control groups. All figures represent the average of three sets of independent experiments.

Fig 5: Drug‐resistant lung cancer cells increased expression of Trps1 and MGMT. (A) The morphology of established H446/CDDP cells. Scale bars: 200 mm. (B) Cells growth curve of H446/CDDP and parental H446 cell. (C) Semi‐QPCR and Western blot analyze the expression of Trps1 and MGMT in H446 and H446/CDDP cells. *P < 0.05 versus H446 control; (D) immunofluorescence assay the expression of Trps1 and MGMT in H446 and H446/CDDP cells. Scale bars: 50 mm. All figures represent the average of three sets of independent experiments.