Fig 2: Immunophenotyping of canine PBL subtypes (representative sample) with gating strategies for total lymphocytes (SSC/FSC) (A), CD3+ T lymphocytes (B), CD3+CD4+ T helper lymphocytes, CD3+CD8+ cytotoxic T lymphocytes, CD3+CD4+CD8+ DP T lymphocytes, CD3+CD4-CD8- DN T lymphocytes (C), CD45+ lymphocytes (D), CD45+CD21+ B lymphocytes (E) are presented. Scatter plots showing CD3+CD4+ T helper lymphocyte and CD3+CD8+ cytotoxic T lymphocyte percentages in healthy controls and dogs in different stages of MMVD (F). aThe median values of the stable and unstable CHF patients were significantly lower (P < .05) than that of the healthy dogs. bThe median values of the stable and unstable CHF patients were significantly higher (P < .05) than that of the healthy dogs. CD, cluster of differentiation; CHF, congestive heart failure; DN T, double negative T; DP T, double positive T; FSC, forward scatter; MMVD, myxomatous mitral valve disease; PBL, peripheral blood lymphocyte; SSC, side scatter