Fig 1: (a) Relative mRNA expression levels of ABCC6 and NT5E gene in Caco2 and HT29 cells. Results are expressed as the 2−ΔCt and are presented as the mean ± sem of at least three independent experiments. β-actin was used as a reference gene. Statistical analysis was performed by 2-way ANOVA followed by Holm–Sidak correction for multiple comparisons. *** p < 0.001. (b) Representative Western blot of the effect of Quercetin and Probenecid on protein expression (D, DMSO 0.25%; Q, Quercetin 165 μM; p, Probenecid 250 μM). mRNA and protein expression levels of NT5E and ABCC6 in Caco2 (c,d) and HT29 (e,f) cells. Result are expressed as the 2−ΔCt and are presented as the mean ± sem of at least three independent experiments. β-actin was used as a reference gene. Statistical analysis was performed by 2-way ANOVA followed by Dunnett’s correction for multiple comparisons. * p < 0.05, ** p < 0.01. Cells treated with vehicle only (DMSO 0.25%) were used as a control, and β-actin was used as a loading control. Data are presented as the mean ± sem of three independent experiments. Statistical analysis was performed by one-way ANOVA followed by Dunnett’s correction for multiple comparisons. * p < 0.05.