Fig 1: The influence of miR-320a and SMG1 on ovarian cancer progression under curcumin exposure. A and B SMG1 expression was detected in SKOV3 and A2780 cells with transfection of inhibitor NC, miR-320a inhibitor, miR-320a inhibitor + si-NC or si-SMG1. Colony-formation ability (C), apoptosis (D), and protein levels of c-caspase-3, PCNA and Bax (E) were measured in SKOV3 and A2780 cells with transfection of inhibitor NC, miR-320a inhibitor, miR-320a inhibitor + si-NC or si-SMG1 after exposure to curcumin. *P < 0.05
Fig 2: The influence of circ-PLEKHM3/miR-320a axis on SMG1 expression in ovarian cancer cells. A-D SMG1 abundance was detected in cells transfected with vector, oe-circ-PLEKHM3, oe-circ-PLEKHM3 + mimic NC or miR-320a mimic. *P < 0.05
Fig 3: SMG1 was a target of miR-320a. A and B SMG1 level was measured in tumor and normal tissues (n = 35). C and D SMG1 level was examined in SKOV3, A2780 and IOSE-80 cells. E The linear correlation of miR-320a and SMG1 in ovarian cancer tissues was analyzed. F and G SMG1 abundance was detected in SKOV3 and A2780 cells after exposure to various concentrations of curcumin. H The binding site of miR-320a and SMG1 was predicted via starBase. I Luciferase activity was detected in 293 T cells transfected with SMG1 3’UTR wt or mut and miR-320a mimic or mimic NC. J The interaction between miR-320a and SMG1 was analyzed by RIP assay. K-L SMG1 abundance was measured in SKOV3 and A2780 cells transfected with si-NC or si-SMG1. *P < 0.05
Supplier Page from Abcam for Anti-Smg1 antibody [EPR4591(2)]