Fig 2: Validation of the expression of TFRC, INTS4, FGG and ATP5B by western blot analysis. The serum levels of TFRC, INTS4, ATP5B and FGG were determined by western blot analysis prior to MI model establishment (0 h) and following MI model establishment at 1, 2 and 3 h (mean ± standard error of the mean, n=10). P-values of multiple groups was calculated using one-way analysis of variance, and pairwise comparison within was performed using the Least Significant Difference test. (A) Representative bands of different proteins at different time points. (B) Relative expression of TFRC at the three time points, 1 h/0 h: 0.95±0.12 (P>0.05); 2 h/0 h: 1.03±0.10 (P>0.05), 3 h/0 h: 1.05±0.10 (P>0.05). (C) Relative expression of ATP5B at the three time points, 1 h/0 h: 0.58±0.06 (P<0.01); 2 h/0 h: 0.56±0.09 (P<0.01), 3 h/0 h: 0.35±0.08 (P<0.01). (D) Relative expression of FGG at the three time points, 1 h/0 h: 1.07±0.21 (P>0.05); 2 h/0 h: 1.72±0.21 (P<0.01), 3 h/0 h: 1.51±0.18 (P<0.01). (E) Relative expression of INTS4 at the three time points, 1 h/0 h: 2.12±0.41 (P<0.01); 2 h/0 h: 1.99±0.35 (P<0.01), 3 h/0 h: 2.20±0.25 (P<0.01). **P<0.01, vs. 0 h/0 h group; #P<0.05 and ##P<0.01, vs. 1 h/0 h group; &P<0.05, vs. 2 h/0 h group. MI, myocardial infarction; ATP5B, ATP synthase F1 subunit ß; FGG, fibrinogen ? chain; INTS4, integrator complex subunit 4; TFRC, transferrin receptor 1.

Fig 3: MTLN Interacts with the Mitochondrial Energetics Machinery(A) Mass spectrometric profiling of co-immunoprecipitated proteins bound to MTLN-FLAG in undifferentiated MPCs and hPSC-adipocytes. Red, MTLN; blue, ATP5B and HADHB. Trendline represents the linear regression of these data using a non-linear model. Data based on two independent biological replicates each of MPCs and WAT. Data in Supplemental Table 2.(B) DAVID gene ontology analysis of the cellular compartment categorization of co-immunoprecipitated proteins bound to MTLN-Flag. Orange, mitochondrial localization; white, protein translation machinery; gray, other. Modified Fisher exact p value, EASE score. Data in Supplemental Table 3.(C) Immunoblot of HADHB co-immunoprecipitated with MTLN-FLAG. Whole-cell lysate is shown as input material.(D) Immunoblot of ATP5B co-immunoprecipitated with MTLN-FLAG. Whole-cell lysate is shown as input material.(E) DAVID gene ontology analysis of the cellular compartment categorization of co-immunoprecipitated proteins bound to MTLN-Flag in hPSC-adipocytes but not MPCs. Orange, mitochondrial localization; white, protein translation machinery; gray, other. Modified Fisher exact p value, EASE score.(F) Gene ontology analysis describing the biological pathways enriched in the co-immunoprecipitated proteins bound to MTLN-Flag in hPSC-adipocytes but not in MPCs. Orange, mitochondrial processes; white, protein translation and overexpression; gray, other. Pathways and processes are colored through personal interpretation of DAVID terms. Modified Fisher exact p value, EASE score.Related to Figure S4.