Fig 2: Expression of Dcn and Tpm1 in cultured mouse lens epithelial cells (MLECs) and human mouse lens epithelial cells (HLECs) after treatment with FGF2 with/without TGFβ-2. (A) Cultured MLECs were plated in 35-mm dishes at the density of 1 × 105 in Dulbecco’s modified Eagle’s media (DMEM) with 10% FBS for 24 h. LECs were treated with 10 ng/mL TGFβ-2 and/or 100 ng/mL FGF2 in DMEM containing 1% FBS for 2 and 4 days. The relative quantity of Dcn mRNA was determined using RT-qPCR analysis. * p < 0.004, ** p < 0.001. (B) Cultured HLECs were plated in 35-mm dishes at the density of 8 × 104 in DMEM with 20% FBS for 24 h. LECs were treated with 0–100 ng/mL FGF2 in DMEM containing 1% FBS for 2 days. Relative quantity of Dcn and Tpm1 mRNA was determined using RT-qPCR analysis. * p < 0.004, ** p < 0.05. Data were from three experiments and were reported as mean ± S.D. M: marker.

Fig 3: Detection of effects of ADSCs on protein expressions of α-SMA and DCN in fibroblasts via immunofluorescence assay.

Fig 4: Detection of effects of ADSCs on mRNA expressions of a-SMA and DCN in fibroblasts via RT-qPCR. (A) mRNA expression of DCN. (B) mRNA expression of a-SMA; **p<0.01.

Fig 5: Relationship between age and Dcn mRNA expression in human LECs, and between age and concentration of DCN protein in human aqueous humor. RT-qPCR validation of Dcn mRNA expression in human LECs (A) and ELISA for DCN concentration in aqueous humor from patients with cataract (B) compared to that in the control (human LECs obtained from the clear lens after vitrectomy for removal of epiretinal membrane). N.S.: not significant; yrs: years-old.