Fig 2: ANLN-210 interacts with HNRNPC.A The knockdown efficiency of si-HNRNPC, si-SRSF10, and si-U2AF2 was assessed at protein level by western blot. B The RNA levels of ANLN-201 or ANLN-210 were measured in si-HNRNPC, si-SRSF10, and si-U2AF2 transfected cells by qRT-PCR. *P < 0.05, **P < 0.01. C The RNA stability of ANLN-210 was evaluated in si-HNRNPC or si-SRSF10 or si-U2AF2 treated with actinomycin D after 4, 8, and 12 h. *P < 0.05, **P < 0.01. D Schematic representation of the ANLN-210 WT and ANLN-210 MUT sequences used as probes in EMSA. MUT: The binding sequences between ANLN-210 and HNRNPC were mutant. ANLN-210 MUT could not interact with HNRNPC. E SCC-9 cells were transfected with HNRNPC in a dose-dependent manner. EMSA was performed using ANLN-210 WT probe (left) and ANLN-210 MUT probe (right).