Fig 1: Camk1g distribution and regulation following dexamethasone (DEX) injection and restraint stress. (a) In situ hybridization autoradiograms depicting the brain distribution of Camk1g mRNA and its up-regulation by dexamethasone (DEX). The animals were sacrificed 4 h after the administration of DEX (16 mg/kg, i.p.) or saline (SAL). Optical densities of the autoradiograms, expressed in photostimulated luminescence units/mm2 (PSL/mm2), have been color-coded according to the scale in the upper right corner. The images are representative of 4 animals per group. (b) The plots show the results of a quantitative hybridization signal analysis in selected brain regions. The data, presented as mean ± SEM (n = 4), were analyzed by the unpaired two-tailed t-tests. *—p < 0.05; ***—p < 0.001. (c) Changes in Camk1g mRNA abundance levels in the amygdala of 5 mice per group after restraint stress compared with a housekeeping gene (Hprt1). The heatmap shows a z-scored abundance of log2-transformed counts for each gene as measured with microarray profiling. T-test p-value: Camk1g = 0.002; Hprt1 = 0.46. Abbreviations: BNST—bed nucleus of stria terminalis; CeA—central nucleus of amygdala; CPu—caudate/putamen; LS—lateral septum; NAc-c—nucleus accumbens core; NAc-s—nucleus accumbens shell; VMH—ventromedial hypothalamic nucleus.