Fluorescent Quenching analysis was performed by loading 1 µg of purified TAMRA-conjugated BSA (T-BSA) or BSA alone (BSA) into a 96 well plate. Protein was then bound to 10 µg of either Mouse IgG whole molecule (Product # 31202) or quenching antibody Anti-TAMRA clone # 11H10 (Product # MA1-042) and compared to unbound TAMRA-BSA. Emissions were read using the Tecan Safire at 550 nm excitation wavelength and reported in RFU (relative fluorescent units).