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Antibodies
Antibodies
Anti-PCK1/PEPC antibody
Anti-PCK1/PEPC antibody from Abcam
Be the first to write a review!
Citations:
(21)
Supplier Page
Supplier Page from
Abcam for
Anti-PCK1/PEPC antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-PCK1/PEPC antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab28455
Antibody Type
Primary
Host
Rabbit
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity
100 UL
Clonality
Polyclonal
Isotype
IgG
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
PCK1
Reactivity
Human, Monkey, Rat
Target
PCK1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (21)
(1)
An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer
Cell Communication and Signaling
December 1, 2022
Rui Zhang, Guo-Jun Geng, Jian-Guang Guo, Yan-Jun Mi, Xiao-Lei Zhu, Ning Li, Hong-Ming Liu, Jun-Feng Lin, Jian-Weng Wang, Guang Zhao,
et al.
(all from
Abcam
; Cat#
ab115730
,
ab234440
,
ab191071
,
ab188317
,
ab38007
,
ab184169
,
ab99170
,
ab133551
,
ab28455
,
ab14715
, and
ab210702
, respectively), anti-
GAPDH
anti-Lamin B, anti-actin and anti-Calnexin (all from
(2)
Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A
International Journal of Molecular Sciences
September 27, 2022
Pavel I. Nedvetsky, Ivo Cornelissen, Thomas Mathivet, Claire Bouleti, Phalla Ou, Pieter Baatsen, Xiaocheng Zhao, Frans Schuit, Fabio Stanchi, Keith E. Mostov,
et al.
immunoblotted with specific antibodies: rabbit anti-albumin (
Bio-Rad
; #
AHP1478
); rabbit anti-Pck1 (
Abcam
;
ab28455
); or rabbit anti-GAPDH (#
2118
;
Cell Signaling Technologies
, Danvers, MA, USA). 4.5.
Scanning Electron Microscopy
(3)
LKB1 acts as a critical brake for the glucagon‐mediated fasting response
Hepatology Communications
August 1, 2022
Suehelay Acevedo‐Acevedo, Megan L. Stefkovich, Sun Woo Sophie Kang, Rory P. Cunningham, Constance M. Cultraro, Natalie Porat‐Shliom
β‐tubulin (
2146S
,
1:1000
;
Cell Signaling Technologies
); phosphoenolpyruvate carboxykinase 1 (PCK1 [
ab28455
],
1:1000
,
Abcam
); glyceraldehyde 3‐phosphate dehydrogenase (
2118S
,
1:1000
;
Cell Signaling Technologies
);
(4)
DA-1241, a Novel GPR119 Agonist, Improves Hyperglycaemia by Inhibiting Hepatic Gluconeogenesis and Enhancing Insulin Secretion in Diabetic Mice
Diabetes & metabolism journal
March 1, 2022
Kim Y, Lee SW, Wang H, Kim RH, Park HK, Lee H, Kang ES.
6-phosphatase (G6Pase;
ab83690
,
Abcam
, Cambridge, UK), phosphoenolpyruvate carboxykinase (PEPCK;
Abcam
,
ab28455
), LC3 (
Sigma
,
L7543
) and
p62
(
sc-28359
,
Santa Cruz
, Santa Cruz, CA, USA), and GFP (
Abcam
,
ab13970
).
(5)
Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas
Scientific Reports
December 1, 2021
Qiguang Wang, Wenke Liu, Si Zhang, Zuoyu Liang, Linhong Jiang, Aiqin Xue, Xiaobo Cen, Qian Bu
(PA5-27,452; Invitrogen), anti-D2-40 (MA1-83,884; Invitrogen), antiCA9 (
ab184006
;
Abcam
), anti-PCK (
ab28455
;
Abcam
), anti-Oligo2 (
ab109186
;
Abcam
), anti-SOX9 (#82,630; Cell signaling technology), anti-Inhibin
(6)
Porphyromonas gingivalis induces entero-hepatic metabolic derangements with alteration of gut microbiota in a type 2 diabetes mouse model
Scientific Reports
December 1, 2021
Yoichiro Kashiwagi et al.
for western blotting
.
Immunoblotting
was performed using the following primary antibodies: PCK1 (
1:1000
;
ab28455
,
Abcam
, Toronto, Canada), G6PC (
ab83690
;
Abcam
),
FOXO1
(
1:000
;
2880
,
Cell Signaling Technology
, Danvers,
(7)
Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
eLife
March 10, 2020
Ma R, Martínez-Ramírez AS, Borders TL, Gao F, Sosa-Pineda B.
#:
1735–01
; RRID:AB_2795211,IF (
1:100
) Antibody,anti-
mouse
PCK1 (rabbit polyclonal),
Abcam
,Cat. #:
ab28455
; RRID:AB_777191,
IHC
(
1:100
) Antibody,anti-
mouse
Beta-gal (
chicken
polyclonal),
Abcam
,Cat. #:
ab9361
;
(8)
Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis
Cell metabolism
January 7, 2020
Huangyang P, Li F, Lee P, Nissim I, Weljie AM, Mancuso A, Li B, Keith B, Yoon SS, Simon MC.
for IHC
,
Sigma-Aldrich
,#
HPA012513
Mouse
polyclonal anti-
FBP1
,
Sigma-Aldrich
,#
SAB1405798
Rabbit polyclonal anti-PCK1,
Abcam
,#
ab28455
Rabbit polyclonal anti-G6PC,
Abcam
,#
ab83690
Rabbit monoclonal anti-GAPDH,
Cell Signaling Technology
,#
2118
Mouse
(9)
Cold acclimation enhances UCP1 content, lipolysis, and triacylglycerol resynthesis, but not mitochondrial uncoupling and fat oxidation, in rat white adipocytes
American Journal of Physiology-Cell Physiology
March 1, 2019
Diane M. Sepa-Kishi, Shailee Jani, Daniel Da Eira, Rolando B. Ceddia
kinase (GYK) (cat. no.
ab180525
), phosphoenolpyruvate carboxykinase cytoplasmic (PEPCK-C; cat. no.
ab28455
), and UCP1 (cat. no.
ab23841
) were purchased from
Abcam
(Toronto, ON, Canada). Animals. Male albino
(10)
Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase
Nature medicine
September 1, 2018
Hunter RW, Hughey CC, Lantier L, Sundelin EI, Peggie M, Zeqiraj E, Sicheri F, Jessen N, Wasserman DH, Sakamoto K.
and FBP1 (
sc-32435
) were from
Santa Cruz Biotechnology
.
PFKL
(
ab181064
),
PKLR
(
ab171744
) and PEPCK-C (
ab28455
) were from
Abcam
.
GAPDH
(
G8795
) and α-tubulin (
T6074
) were from
Sigma
.
SLC22A1
/
OCT1
(#
ACT-011
) antibody
(11)
Metabolic reprogramming by PCK1 promotes TCA cataplerosis, oxidative stress and apoptosis in liver cancer cells and suppresses hepatocellular carcinoma
Oncogene
March 1, 2018
Meng-Xi Liu et al.
purchased from Sigma-Aldrich, and dichlorofluorescin diacetate from Beyotime. Antibodies against PCK1 (
ab28455
,
Abcam
), PCK2 (
ab70359
,
Abcam
) and YAP1 (
ab52771
,
Abcam
), phospho-AMPKα (Thr172) (
Cell Signaling Technology
,
(12)
O-GlcNAcylation mediates the control of cytosolic phosphoenolpyruvate carboxykinase activity via Pgc1α
PloS one
January 1, 2017
Latorre P, Varona L, Burgos C, Carrodeguas JA, López-Buesa P.
(Sigma) and 10% skimmed milk for 1 h. The
membrane
was incubated overnight at 4°C with Anti-PCK1 (
Abcam
Ab28455
,
1:500
) and anti-actin (
Sigma
A2066
1:20000
) and then washed and incubated with secondary HRP-conjugated
(13)
Comparative proteomic profiling identifies potential prognostic factors for human clear cell renal cell carcinoma
Oncology Reports
November 1, 2016
Xiang Sun, Hongwei Zhang, Longhua Luo, Kezhao Zhong, Yushui Ma, Linlin Fan, Da Fu, Lijuan Wan
transferred to a PVDF
membrane
and probed with the following polyclonal antibodies: anti-PCK1 and
anti-SNRPF
(
ab28455
and
ab156587
,
1:500
;
Abcam
).
GAPDH
(
sc-48166
,
1:1,000
;
Santa Cruz Biotechnology, Inc
.) was used as a
(14)
Fructose-1,6-bisphosphatase opposes renal carcinoma progression
Nature
September 11, 2014
Li B, Qiu B, Lee DS, Walton ZE, Ochocki JD, Mathew LK, Mancuso A, Gade TP, Keith B, Nissim I,
et al
.
detecting HIF1α (
NB100-134
) and HIF2α (
NB100-122
) were obtained from
Novus
, and antibodies against PCK1 (
ab28455
), G6PC (
ab83690
), and PFKL (
ab37583
) were from
Abcam
. D-fructose-1, 6-bisphosphate trisodium salt (47810),
(15)
The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice
PloS one
January 1, 2014
Jakobs HH, Mikula M, Havemeyer A, Strzalkowska A, Borowa-Chmielak M, Dzwonek A, Gajewska M, Hennig EE, Ostrowski J, Clement B.
St. Louis, USA), anti-CYB5B-antibody (
HPA007893
;
Sigma-Aldrich
, St. Louis, USA), anti-PCK1-antibody (
ab28455
;
Abcam
, Cambridge, UK), anti-MOSC1-antibody (
AP9754c
;
Abgent
, San Diego, USA) for of HepG2 lysates or
(16)
Effects of maternal nutrient restriction, intrauterine growth restriction, and glucocorticoid exposure on phosphoenolpyruvate carboxykinase-1 expression in fetal baboon hepatocytes in vitro
Journal of medical primatology
August 1, 2013
Li C, Shu ZJ, Lee S, Gupta MB, Jansson T, Nathanielsz PW, Kamat A.
(17)
Suppression of Gluconeogenic Gene Expression by LSD1-Mediated Histone Demethylation
PLoS ONE
June 5, 2013
Dongning Pan, Chunxiao Mao, Yong-Xu Wang
against LSD1 (
Abcam
#
ab17721
), FBP1 (
Abgent
#
AP7385C
), G6Pase (
Santa Cruz
#
sc-25840
), PEPCK (
ABcam
#
ab28455
) and β-tubulin (DSHB #E7-S) were used. RNA isolation and real time
quantitative PCR
Total RNA was
(18)
The histone demethylase Jhdm1a regulates hepatic gluconeogenesis
PLoS genetics
January 1, 2012
Pan D, Mao C, Zou T, Yao AY, Cooper MP, Boyartchuk V, Wang YX.
were separated by
SDS-PAGE
and probed with antibody against C/EBPα (
Santa Cruz
,
sc-61
), PEPCK (
ABcam
,
ab28455
) or G6Pase (
Santa Cruz
,
sc-25840
).
Co-immunoprecipitation
HA-Jhdm1a and Flag-USF1 plasmids were co-
transfected
(19)
Inhibition of glyceroneogenesis by histone deacetylase 3 contributes to lipodystrophy in mice with adipose tissue inflammation
Endocrinology
May 1, 2011
Zhang J, Henagan TM, Gao Z, Ye J.
(20)
Epigenetic modification of fetal baboon hepatic phosphoenolpyruvate carboxykinase following exposure to moderately reduced nutrient availability
The Journal of physiology
April 15, 2010
Nijland MJ, Mitsuya K, Li C, Ford S, McDonald TJ, Nathanielsz PW, Cox LA.
antibody overnight at 4◦ C. The primary antibody for PCK-1 was used at final dilution of
1:3000
(cat.
ab28455
;
Abcam
, Cambridge, MA, USA) using the standard
IHC
peroxidase
technique
with Elite ABC kits (cat.
PK-6100
,
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Reviews
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Anti-PCK1/PEPC antibody from Abcam
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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