Fig 1: Validation of One-month-old Mouse Heart RNA-Sequencing Results by qRT-PCR and Western Blot. Samples (RNAs and proteins) being analyzed were extracted from WT and Lmna-/- mouse heart tissues at 1 month of age. (a) RT-qPCR validation of down-regulated genes (ACDVL, ATP5G1, CRAT, ECH1, KCDN2, P2RY1, PPARA) identified by RNA-Sequencing. The same ??Ct method was used for analysis as described in Fig. 3 legends. The relative expression levels of all the listed genes are showed as “mean ± SEM” on Y axis. The expression levels of all the listed genes among 1-month Lmna-/- mouse hearts were significantly lower than the levels among 1-month WT mouse hearts (p < 0.05). (b) RT-qPCR validation of up-regulated genes (DUSP4, LOX, FHL1, MYOM2, NMRK2) identified by RNA-Sequencing. The same ??Ct method was used for analysis. The relative expression levels of all the listed genes are showed as “mean ± SEM” on Y axis. The expression levels in 1-month Lmna-/- mouse hearts were significantly higher than those in 1-month WT mouse hearts for all the genes (p < 0.05). (c) Western blot validation of protein expressions of representative down-regulated (ECH1 and PPARA) and up-regulated (DUSP4 and FHL1) genes. ß-actin (for ECH1 and PPARA) or GAPDH (for DUSP4 and FHL1) was used as a loading control. Three repeated experiments were conducted for each protein with similar results showed in the figure.