Fig 2: IHC of WT and G93A mutant SOD1 lumbar spinal cord for BH3-only protein expression in motor neurons. (A) IHC of Bik expression in motor neurons of G93A and WT lumbar spinal cord tissue. Lumbar spinal cord was sagittally cut 8 μm thick onto standard glass slides. Slides were stained and imaged for Bik (Cy3, red) and DAPI (blue). Arrows point to large Bik-positive motor neurons. The image shown is representative of the results observed with other BH3-only proteins, Bim, Noxa, Puma, and Bad. (B) Quantification of BH3-only positive motor neurons. Data shown represent the mean ± SEM for the number of BH3-only positive motor neurons quantified from five 40X fields per animal. Mean and SEM were calculated from N = 5–7 WT and G93A mice per BH3-only protein. When analyzed by unpaired t-test, no significant differences in the number of BH3-only positive motor neurons were found between WT and G93A mutant SOD1 lumbar spinal cord sections for any of the BH3-only proteins analyzed.