Fig 1: CRP diminishes the antigen presenting ability of moDCs though Fc?R2B. (A) The mRNA expression levels of MHC-II, CD86, PDL-1, and OX40L were measured in LPS and LPS CRP samples from WT moDCs by qPCR (n = 6). (B, D) moDCs were established from WT mice and CRP-/- mice, then incubated with or without CRP and LPS. The expression of MHC-II and CD86 was analyzed by flow cytometry and the MFI was recorded (n = 4). (C, E) moDCs were established from Fc?R2B-/- mice and cultured with or without CRP and LPS, then the expression of MHC-II and CD86 was analyzed by flow cytometry and the MFI was recorded (n = 4). Data are presented as mean ± SEM; p < 0.05 was considered statistically significant. ns, no significance.