Fig 1: BoTA-induced endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in a thin endometrium mouse model A Venn diagram of DEGs from EtOH-treated vs. saline-treated uteri (GSE207379) and BoTA-treated vs. saline-treated (GSE146934) displaying a shared gene with a reciprocal expression; Igfbp3. B Gene–gene network analysis of DEGs of EtOH-treated vs. saline-treated uteri categorized in CC GO terms displaying a shared gene; Spp1 C Immunoblotting analysis of IGFBP3 and OPN in BoTA-treated thin endometrium compared to saline-treated group. D Immunoblotting analysis of OPN in BoTA-treated thin endometrium compared to IGFBP3-treated thin endometrium. ß-actin was used as a loading control. E An experimental schedule for an application of IGFBP3 neutralizing antibody together with BoTA. F Immunoblotting analysis of IGFBP3 and OPN in response to IGFBP3 neutralizing antibody treatment. ß-actin was used as a loading control. G Immunostaining of IGFBP3 in saline-treated, BoTA-treated, and BoTA with IGFBP-3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of IGFBP3 in the whole region of uterus and the indicated region with a box is magnified in lower panel. Scale bar; 100 µm. H Immunostaining of active-OPN in saline-treated, BoTA-treated, and BoTA with IGFBP3 neutralizing antibody-treated thin endometrium. Upper panel shows the expression of active-OPN in the whole region of uterus and the indicated regions (LE luminal epithelium, GE glandular epithelium) with boxes are magnified in lower panel. Scale bar: 100 µm. I H&E images for saline-, BoTA-, and BoTA with IGFBP3 neutralizing antibody-treated uteri. Scale bar: 100 µm