Fig 1: Compound 21c induces HMGCR degradation through VHL-dependent ubiquitin–proteasome system in Insig-silenced HepG2 cells. (A) Knockdown efficiency of Insig-1 and Insig-2 in HepG2 cells was determined by Western blotting. (B) and (C) Insig-silenced HepG2 cells treated with lovastatin or 21c at the indicated doses (16 h), were examined. (D) and (E) Cells pretreated for 6 h with VH032 (10 μmol/L), the proteasome inhibitor MG-132 (10 μmol/L), lovastatin (3 μmol/L) or DMSO, were subsequently treated for 10 h with compound 21c (1 μmol/L).
Supplier Page from Abcam for Anti-Insig1 antibody