Fig 1: Correlation between B7-H4 and p-PKCd levels. A TCGA dataset analysis was performed on the GEPIA website (http://gepia.cancer-pku.cn/). This analysis showed that PRKCD (PKCd) mRNA expression was positively correlated with VTCN1 (encoding B7-H4) mRNA expression. B The protein levels of B7-H4 and p-PKCd in the NCM460, SW480, HCT116, SW620 and RKO cell lines were determined by Western blot analysis. The data are expressed as the mean ± SD values; n = 3. C Serial p-PKCd+ tumor sections were examined for B7-H4 expression, and positive staining (400 ×) of B7-H4 and p-PKCd in CRC samples is shown. Double IF staining of CRC samples was performed. CRC tissue samples were stained for p-PKCd (green, 200 ×) and B7-H4 (red, 200 ×). B7-H4+/p- PKCd+ cells were identified in CRC tissue specimens (dark yellow; original magnification). D TCGA COAD dataset analysis was performed on the XENA website. This analysis showed that the B7-H4 mRNA level was positively correlated with lymph node metastasis