Fig 2: Aberrant TBK1 accumulation depends on TAX1BP1. (A) fip200 KO, FIP200 WT, and FIP200 ?CT MEFs were transfected with either 20 nM negative control siRNA or 20 nM Tax1bp1 siRNA for 72 h, and cleared cellular lysates were immunoblotted for the indicated proteins. The FIP200 cDNAs used for reconstitution of fip200 KO MEFs encoded an N-terminal FLAG peptide. The densities of the bands on immunoblots from three independent experiments were quantified and normalized to those of Actin. The full-length blots are presented in Figure S7. (B) To compare basal expression levels between the cell lines used, the normalized densities of the untransfected samples were normalized to the signal of FIP200 WT MEFs. (C) To analyze the influence of siRNA transfection, the normalized densities of all samples of each cell line were normalized to that of the corresponding untransfected sample. Only the relative levels of TAX1BP1, TBK1 pS172, and SQSTM1/p62 pS403 are shown. The boxes represent the highest and the lowest value, while the centerline shows the mean. Two-way ANOVA with uncorrected Fisher's LSD test was used to determine differences between treatments. n.s. = not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.