Fig 2: TGFBRAP1 plays a critical role in CHIT1 augmentation of TGF-ß1 signaling and TGFßR expression.(A) Western blot evaluation on the NHLF cells stimulated by rTGF-ß and/or rCHIT1 with (+) and without (-) siRNA silencing of TGFBRAP1. Scrambled siRNA was used as control for TGFBRAP1 specific siRNA. (B) Evaluation on the SMAD activities on the cells transfected SMAD2-luciferase construct after stimulated by rTGF-ß (20 ng/ml) and/or rCHIT1 (100 or 200 ng/ml) with and without siRNA silencing of TGFBRAP1. Panel A is a representative immunoblot of three separate experiments. Values in panel B represent the mean ± SEM of triplicated evaluations in a minimum of two separate experiments. *P < 0.05, **P < 0.01, one-way ANOVA.