Fig 2: Gene expression and immunofluorescence staining of the pancreasGene expression by real-time PCR for (A) glucagon (gcg), (B) GLP-1 receptor (glp1r), and (C) insulin receptor (ins1). (D) Fluorescent immunohistochemistry in the pancreas for insulin (red) and glucagon (green). Micrometer is 50 μm. Photos taken at 20×. (E) Total area of the islet, which includes insulin and glucagon producing cells. (F) Total area of the β-cell, which includes insulin producing cells. (G) Total area of the α-cells, which includes glucagon producing cells. Data are presented as mean ± SEM. Analysis was determined by two-way analysis of variance for main effects; *P<0.05, **P<0.01. Within-group analyses were performed using Student’s t-test. *P<0.05, Scale bar: 25 µms.

Fig 3: Plasma Hormones measured from terminal plasma 16 weeks post-injury(A) C-Peptide (B) Leptin (C) GIP (D) Glucagon (E) GLP-1 (F) DPP4. Data are presented as mean ± SEM. Analysis of each hormone was determined by two-way analysis of variance for main effects of injury and diet; P<0.05, P<0.01.