Purified anti-His Tag 652502 from BioLegend

Product Specs
ItemPurified anti-His Tag
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number652502
Quantity100 µg
ApplicationsWestern Blot (WB), Immunocytochemistry (ICC)
Conjugate/TagUnconjugated
ReactivityEpitope tag
IsotypeIgG1, Kappa
CloneJ099B12
HostMouse
Concentration0.50 mg/mL
FormatThis antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3
ImmunogenKLH-conjugated His-tag peptide
ClonalityMonoclonal
Antibody TypePrimary
TargetHis Tag

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Designing epitope-focused vaccines via antigen reorientationbioRxivDecember 24, 2022Duo Xu, Chunfeng Li, Ashley Utz, Payton A.B. Weidenbacher, Shaogeng Tang, Mrinmoy Sanyal, Bali Pulendran, Peter S. KimExpi-293F cells via transient transfection. Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG, HRP conjugated (clone Poly4053, BioLegend #405306), goat anti-mouse IgG1-HRP

(2) SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genesNature CommunicationsDecember 1, 2022Vladislav N. Nikolov, Dhara Malavia, Takashi KubotaHis-Rtt106 and Rtt106-6HA were detected using anti-FLAG M2-HRP (1:5000, A8592, Merck), anti-His (1:5000, 652502, Biolegend), and anti-HA (1:5000, HA.11 clone 16B12, Biolegend) antibodies, respectively. Spot assay Yeast

(3) Soluble Human Angiotensin- Converting Enzyme 2 as a Potential Therapeutic Tool for COVID-19 is Produced at High Levels In Nicotiana benthamiana Plant With Potent Anti-SARS-CoV-2 ActivityFrontiers in Plant ScienceDecember 6, 2021Tarlan Mamedov, Irem Gurbuzaslan, Damla Yuksel, Merve Ilgin, Gunay Mammadova, Aykut Ozkul, Gulnara Hasanovaquantify the expression levels of ACE2 and ACE2 proteins. Purified anti-His Tag antibody (Cat. No. 652502, BioLegend) was used as primary and mouse IgG used as secondary antibodies to detect ACE2 proteins.

(4) In vivo characterization of emerging SARS-CoV-2 variant infectivity and human antibody escape potentialCell reportsOctober 19, 2021Lam B, Kung YJ, Lin J, Tseng SH, Tsai YC, He L, Castiglione G, Egbert E, Duh EJ, Bloch EM, et al.antibody,Invitrogen,Catalog # 31402 RRID: AB_228395 Anti-His secondary antibody,BioLegend,Catalog # 652502; RRID: AB_2556553 Anti-VSV-G,ATCC,Catalog # CRL-2700 ,, Bacterial and virus strains,Bacterial and

(5) Nasally Administered Lactococcus lactis Secreting Heme Oxygenase-1 Attenuates Murine EmphysemaAntioxidants (Basel, Switzerland)October 27, 2020Yumoto K, Sato T, Nakashima K, Namai F, Shigemori S, Shimosato T, Kaneko T.(OSA-110; ENZO Life Sciences, Farmingdale, NY, USA; 1:1000 dilution, at 4 °C overnight), anti-His tag (652502; BioLegend, San Diego, CA, USA; 1:1000 dilution), or β-actin (A1978; Sigma; 1:10,000 dilution, at room

(6) Noncompetitive Allosteric Antagonism of Death Receptor 5 by a Synthetic Affibody LigandBiochemistryOctober 13, 2020Vunnam N, Szymonski S, Hirsova P, Gores GJ, Sachs JN, Hackel BJ.twice with PBS to remove the unbound soluble proteins and labeled with mouse His6-antibody (Biolegend, 652502), followed by AlexaFluor555-conjugated anti-mouse secondary antibody (Invitrogen, A32727). Colocalization

(7) Recruitment of CD103⁺ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapyScience advancesDecember 1, 2019Williford JM, Ishihara J, Ishihara A, Mansurov A, Hosseinchi P, Marchell TM, Potin L, Swartz MA, Hubbell JA.

(8) A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursorThe Journal of cell biologyFebruary 4, 2019Liu L, Ding L, Rovere M, Wolfe MS, Selkoe DJ.AB_398291; BD Biosciences); for TfR, 612124 (mouse, RRID: AB_399495; BD Biosciences), and for His-tag, 652502 (mouse, RRID: AB_11204427; BioLegend). Electrophoresis and WB Samples were loaded onto 4–12% or 8%

(9) Engineering, and production of functionally active human Furin in N. benthamiana plant: In vivo post-translational processing of target proteins by Furin in plantsPloS oneJanuary 1, 2019Mamedov T, Musayeva I, Acsora R, Gun N, Gulec B, Mammadova G, Cicek K, Hasanova G.analysis. The His tagged proteins were probed with a purified mouse anti-His tag primary antibody (Cat. no. 652502, BioLegend) and anti-mouse horseradish peroxidase (HRP)-conjugated IgG secondary antibody (Cat. No.

(10) Autoreactive, Low-Affinity T Cells Preferentially Drive Differentiation of Short-Lived Memory B Cells at the Expense of Germinal Center MaintenanceCell reportsDecember 18, 2018Jain RW, Parham KA, Tesfagiorgis Y, Craig HC, Romanchik E, Kerfoot SM.

(11) Structural insights into the function of type VI secretion system TssA subunitsNature CommunicationsDecember 1, 2018Samuel R. Dix et al.HisProbe-HRP conjugate (Thermo Fisher, cat. no. 15165) or mouse anti-KLH-conjugated His-tag peptide (BioLegend, 652501). HRP-linked goat-derived (secondary) antibodies that cross-react to rat and rabbit IgG were obtained

(12) Recombinant Mouse Osteocalcin Secreted by Lactococcus lactis Promotes Glucagon-Like Peptide-1 Induction in STC-1 CellsCurrent microbiologyJanuary 1, 2018Namai F, Shigemori S, Sudo K, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T.

(13) Genetically modified Lactococcus lactis producing a green fluorescent protein-bovine lactoferrin fusion protein suppresses proinflammatory cytokine expression in lipopolysaccharide-stimulated RAW 264.7 cellsJournal of dairy scienceSeptember 1, 2017Shigemori S, Namai F, Yamamoto Y, Nigar S, Sato T, Ogita T, Shimosato T.

(14) In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-β-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatusPloS oneJanuary 1, 2017Mamedov T, Cicek K, Gulec B, Ungor R, Hasanova G.followed by Western blotting. PA83 bands were detected using the anti-4xHis tag mAb (BioLegend, Cat. no. 652502); Ps48/45, Endo H or PNGase F bands were detected using the purified anti-DYKDDDDK tag antibody (anti-FLAG

(15) Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactisApplied Microbiology and BiotechnologyJanuary 1, 2017Suguru Shigemori, Masaki Ihara, Takashi Sato, Yoshinari Yamamoto, Shireen Nigar, Tasuku Ogita, Takeshi Shimosatoblotted-membrane was blocked with skim milk, and then was reacted with mouse anti-His tag antibody (Ab) (1/1,000) (652501; BioLegend, San Diego, CA, USA), followed by incubation with horseradish peroxidase 7 (HRP)-conjugated

(16) Nuclear protein kinase CLK1 uses a non-traditional docking mechanism to select physiological substratesThe Biochemical journalDecember 15, 2015Keshwani MM, Hailey KL, Aubol BE, Fattet L, McGlone ML, Jennings PA, Adams JA.

(17) Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in miceMicrobial Cell FactoriesDecember 1, 2015Suguru Shigemori, Takafumi Watanabe, Kai Kudoh, Masaki Ihara, Shireen Nigar, Yoshinari Yamamoto, Yoshihito Suda, Takashi Sato, Haruki Kitazawa, Takeshi Shimosatomembrane for western blotting. Western blotting was performed with mouse anti-His-tag Ab (1/1000) (652501; BioLegend, San Diego, CA, USA) or rabbit anti-HO-1 Ab (1/1000) (SAB2101053; Sigma-Aldrich, St. Louis,

(18) Effect of virus infection on the inflammatory response. Depression of macrophage accumulation in influenza-infected miceThe American journal of pathologyNovember 1, 1976E S Kleinerman, C A Daniels, R P Polisson, R Snyderman

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Purified anti-His Tag from BioLegend

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Purified anti-His Tag from BioLegend

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