Fig 2: Irradiation induces YB-1 expression in brain tumor initiating cells. (A) R28, R11 and R49 cells were irradiated with 2 Gy. Cell lysates were prepared at the indicated time periods (2-8 h) following irradiation. YB-1 expression was determined by western blot analysis. GAPDH was used as a control. (B) Upregulation of total YB-1 following irradiation at the indicated time points. YB-1, Y-box binding protein-1.

Fig 3: TCLlnc1 provoked lymphoma progression via HNRNPD and YBX1.A Expression of TGFB2 and TGFBR1 after overexpression of TCLlnc1, HNRNPD, or YBX1 detected by qRT-PCR. B Expression of TGFB2 and TGFBR1 after knockdown of TCLlnc1, HNRNPD, or YBX1 detected by qRT-PCR. C Luciferase activity assay of TGFB2 and TGFBR1 promoter in cells transfected with HNRNPD siRNA, YBX1 siRNA, or NC siRNA. D Proliferation of Jurkat cells after transfection of HNRNPD siRNA or the YBX1 siRNA, plasmid encoding TCLlnc1, or control vector. E Migration of Jurkat cells after transfection of HNRNPD siRNA or YBX1 siRNA, plasmid encoding TCLlnc1, or control vector. F A schematic model of lncRNA TCLlnc1 function in PTCL.

Fig 4: Irradiation induces YB-1 nuclear translocation in brain tumor initiating cells. (A) The cells were irradiated with either 2 Gy (R11 and R28 cells) or 6 Gy (R49 cells). At the indicted time periods nuclear extracts were generated. As a control, a cytoplasmic protein extract was prepared. YB-1 expression was detected by western blot analysis. To control extract purity, detection of the nuclear protein histone H3 and the cytoplasmic protein calreticulin was performed. (B-D) Upregulation of the different isoforms of nuclear YB-1 in non-irradiated and irradiated (B) R28, (C) R49 and (D) R11 cells at the indicated time points. YB-1, Y-box binding protein-1; Rtx, irradiation; C, cytoplasmic extract; N, nuclear extract.

Fig 5: YB1 enhances migration and invasion in HCC cells. A. Immunoblotting analysis of YB1, E-cadherin, N-cadherin, and ZEB1 in HCCLM3 and MHCC97H cells stably expressing YB1 shRNAs. Vinculin was used as a loading control. B. Immunoblotting analysis of YB1, E-cadherin, N-cadherin, and ZEB1 in BEL7402 and SMMC7721 cells stably overexpressing YB1. Vinculin and GAPDH was used as a loading control. C-D. The cell migration ability was detected with wound healing assay in MHCC97H cells stably expressing YB1 shRNAs (C) and SMMC7721 cells stably overexpressing YB1 (D; magnification, ×100; Scale bar, 100 µm). E-F. The cell invasion ability was detected with Transwell plates in MHCC97H cells stably expressing YB1 shRNAs (E) and SMMC7721 cells stably overexpressing YB1 (F; magnification, ×100; Scale bar, 100 µm). The most representative results of three independent biological experiments were shown. Data were presented as mean ± SD. *P < 0.05, **P < 0.001, significant