Fig 2: Knockdown of NAA10 inhibits cell proliferation and survival in breast cancer cell lines. A, mRNA expression levels of NAA10, measured by quantitative RT-PCR, in a panel of 21 breast cancer cell lines. mRNA expression levels in the immortalized but nontumorigenic breast epithelial cell line MCF10A cells were arbitrarily set as 1. Cell lines: black, MCF10A; green, normal-like breast cancer line; blue, luminal breast cancer cell lines; pink, HER2+ breast cancer cell lines; and red, basal-like breast cancer cell lines. B, Immunoblot analysis of NAA10 expression in a panel of breast cancer cell lines as well as MCF10A line. C, top panel: western blots showing successful knockdown NAA10 protein levels with 2 different siRNAs. Bottom panel: bar graph shows relative cell growth after knocking down NAA10 in SUM149, MDA-MB-468, and SUM1315 breast cancer cells (*P < .05, **P < .01). Data are expressed as mean ± SD

Fig 3: Lysine acetyltransferases (KATs) associated with disease-free survival of breast cancer and contributed to growth and viability of tumor cells in vitro. A, Kaplan-Meier plots of disease-free survival associated with mRNA expression levels of 4 KATs (ACAT2, BRD4, NAA10, and NCOA3) in Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) breast cancers. For the survival analysis, we equally divided 1980 METABRIC samples into high and low expression groups for each KAT based on mRNA expression Z scores. B, Scatter plot showing mean of each KAT dependency score in genome-scale loss-of-function screens of 712 tumor lines