Fig 1: mRNA and protein expression of AKR1C1 (A, E), P450 (B, F), 3β-HSD (C, G) and 17β-HSD (D, H) in uterine tissues (endometrium and myometrium) on 4th and 13th day of estrous cycle, in pregnancy and anestrus phase. Data were normalized against GAPDH for mRNA expression and against β-actin (ACTB) for proteins expression. Each bar represents one experimental group with SEM. Statistical differences were analyzed by two-way analysis (ANOVA) of variance followed by the Bonferroni post hoc test using GraphPad PRISM (Version 8.3.0). The lowest statistical significance was P < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (*P < 0.1; **P < 0.01; ***P < 0.001; ****P < 0.0001). Different letters indicate statistical differences (P < 0.05) between the experimental groups throughout endometrium (A, B) and myometrium (a–b) respectively.