Fig 1: Expression of GLUT3 and GLUT5 increases in the ipsilateral striatum after ICH. (a) Representative Western blot (left) and quantification of GLUT3 expression (right) in striatum after ICH. n = 4/group. ###p < 0.001. Two-way ANOVA with Bonferroni post hoc test. The membrane was cut before it was hybridized with GLUT3 antibody according to molecular weight of GLUT3. Raw images of the blots are presented in Supplementary Fig. 3. (b) Representative images and quantification of MAP2 expression in the ipsilateral striatum on days 1 and 3 after ICH. Scale bar: 50 µm. Stars in the outlined areas indicate the hemorrhagic core. n = 5/group; *** p < 0.001; one-way ANOVA with Bonferroni multiple comparison test. (c) Representative gating of CD11b-FITC+ / Ly6G-APC+ neutrophils and CD11b-FITC+ / Ly6G-APC- MMI and fold change of GLUT3 mRNA in cells at day 1 after ICH. n = 4, ***p < 0.001 versus sham MMI. ###p < 0.001 versus ICH MMI. Ntr, neutrophils; MMI, macrophage/microglia. (d) Representative images of CD41 expression in the ipsilateral striatum on day 1 after collagenase-induced ICH and autologous blood-induced ICH. Red: CD41. Blue: DAPI. Scale bar: left 100 µm; right 25 µm. (e) Representative Western blot (left) and quantification of GLUT5 expression (right) in striatum after ICH. n = 4/group. ###p < 0.001. Two-way ANOVA with Bonferroni post hoc test The membrane was cut before it was hybridized with GLUT5 antibody according to molecular weight of GLUT5. Raw images of the blots are presented in Supplementary Fig. 3. (f) Brain sections were immunostained for Iba1 (green) and GLUT5 (red) to characterize microglia in the perihematomal region on day 3 after ICH. The area outlined in the lower left corner indicates the hematoma core. The arrow in the lower panel indicates one microglia under higher magnification. Insets show the same microglia under higher magnification. Scale bar: upper panel, 50 µm; lower panel, 25 µm; inset, 10 µm. Graph shows the quantification of GLUT5 expression in the ipsilateral striatum at multiple time points after ICH. n = 5/group. *p < 0.05, ***p < 0.001 versus sham group. (g) Comparison of standardized uptake value ratio on PET scan and relative GLUT1 level evaluated by Western blotting in the ipsilateral striatum at the indicated times after ICH. Pearson correlation test. Correlation coefficient r = 0.845, p = 0.034. Data are presented as mean ± SD at each time point.