Fig 1: RNF213 facilitates recruitment of ubiquitin adaptors to the Toxoplasma PV. (A) Representative confocal images and quantification of ubiquitin adaptors (p62, TAX1BP1, NDP52, and OPTN) and ubiquitin (FK2) colocalization to Toxoplasma Pru PVs in WT A549 cells. Unprimed and IFN-γ-primed (100 U/mL) cells were infected with Toxoplasma Pru at an MOI of 3 for 6 h. Statistical comparisons is shown for groups “both.” (B) Unprimed and IFN-γ-primed (100 U/mL) WT and RN213 KO A549 cells were infected with Toxoplasma Pru at an MOI of 3, and the localization of p62, TAX1BP1, NDP52, and OPTN to Toxoplasma PVs was quantified at 6 hpi. All data depict the mean ± SEM from 3 to 4 independent experiments. Two-way ANOVA followed by Tukey’s multiple-comparison test was used to determine significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.