Fig 1: Examination of redox homeostasis in macrophages during infection. (A) Western blot analysis of protein abundance in BMDMs untreated or infected for 2 h with swollen conidia. Proteins were isolated from macrophages and evaluated via western blot with NOX2 (anti-gp91phox), SOD2, GRX1, or GAPDH antibodies. (B) Quantification of the intensity of corresponding bands on blots as shown on (A) from 3 (NOX2, SOD2) and 2 (GRX1) independent experiments. Images were processed in ImageJ. The integrated density of each band was measured after background subtraction and image inversion. Values were normalised to the integrated density from the corresponding lanes. Data shown in relation to a band intensity for GAPDH obtained in each experiment. Rhombs and error bars indicate means and standard deviation.