Fig 2: AKT1 overexpression significantly increased the AKT1 protein expression level and Ser473 phosphorylation level while reducing FOXO1 protein expression in HUVECs or HAECs with or without ox-LDL treatment (A–G). *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.

Fig 3: Downregulation in the FOXO1 pSer256/total FOXO1 ratio and AKT1 pSer473 level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.