Fig 1: Typical OWLS kinetic curves of Ab deposition and their corresponding fits are shown. (A) Polyclonal Ab (Bio-Rad rabbit 4329-4906 on protein A surface). (B) Monoclonal Ab (Bio-Rad mouse OBT0749 on protein A surface). The smaller adsorbed surface mass density and slower adsorption are clearly visible in the case of the monoclonal Ab.
Fig 2: (A) Testing the binding abilities of different polyclonal Abs with an in-house ELISA test with different amounts of intact E. coli cells as antigen. Black: Bio-Rad 4329-4906, white: Thermo Fisher PA1-7213, grey: Thermo Fisher PA1-73032. (B) The binding ability of a monoclonal Ab (Bio-Rad OBT0749) with an in-house ELISA test with different amounts of intact E. coli cells as antigen. For detection, biotinylated polyclonal Ab (Bio-Rad 4329-4906) was used. The inset shows the schematic drawing of the assay.
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