Fig 1: Role of IFN signalling in melanoma invasion plasticity. (a) Inhibition of Jak1/2 by Ruxolitinib promotes the elongated, mesenchymal phenotype of melanoma cells cultured in 3D collagen (48 h). (b) Representative image of WM3629 cells after 48 h in 3D collagen treated with DMSO or Ruxolitinib. (c) Quantification of morphology of WM3629 cells treated with IFNβ alone or IFNβ plus Ruxolitinib after 48 h in collagen. (d) Quantification of morphology of melanoma cells cultured in 3D collagen for 48 h after treatment with IFNs (overall exposure to IFNs took 96 h). (e) Representative images of WM3629 cells after 48 h in 3D collagen treated with IFNs. (f) Immunoblotting detection of Stat transcription factors Stat1, Stat2 and Stat3 activation after 1 h and 48 h IFN treatment in WM3629 cells. Scale bar 100 µm in both (b) and (e). R = round, E = elongated. p-values: *** p < 0.001, ** p < 0.01, * p < 0.05. Detailed information about Western blot can be found at Figure S4.