Fig 2: Protein corona identification and classification. (A) SDS-PAGE analysis of plasma proteins obtained from BMP-RA-TZ and BMP-GA-TZ following incubation with plasma and IgG plasma. (B and C) Classification based on MW of proteins obtained from BMP-RA and BMP-GA following incubation with plasma and IgG plasma. Average protein MW was higher for BMP-GA-TZ-plasma than for BMP-RA-TZ-plasma. (D) Hydrate particle size analysis of BMPs, BMP-plasma complex, and BMP-IgG plasma complex (n=3). (E) Zeta potential of BMPs, BMP-plasma complex, and BMP-IgG plasma complex (n=3). (F) ELISA analysis of HER2-binding capacity of BMP-RA-TZ and BMP-GA-TZ before and after incubation with plasma and IgG plasma (n=3). *P <0.05; **P <0.01; ***P <0.001; ****P <0.0001.

Fig 3: Conducting 3D-FISH validations of the PRKD3 loop in organoids of BC tissues. A Images of organoids derived from normal breast tissue (NT), primary breast cancer tissue (PT) and tamoxifen-treated breast cancer recurrent tissue (RT). Scale bar at 50 µm. B IHC staining of ER and PR in organoids of NT, PT and RT respectively with a higher magnification (40X) and a scale bar at 50 µm. C Western blotting showing ER, PR, HER2 and Ki67 protein levels in organoids of NT, PT and RT, respectively. ß-actin levels were measured as a loading control. D 3D-FISH images showing interaction frequency of the PRKD3 loop in organoids of PT and RT, respectively. BAC probe combinations: promoter (green) and distal region (red) n = 50, DAPI DNA stain (blue). Square boxes in red represent the magnified view of each interaction. Scale bar at 5 µm. E Distributions of measured distances of the PRKD3 loop are significantly different for PT vs. RT (*p = 0.1) by one-tail paired t test analysis. Distances were measured between the closest two foci in each nucleus

Fig 4: Glutaraldehyde (GA) and RA complexes with TZ/BMP (BMP-GA-TZ, BMP-RA-TZ). (A) SDS-PAGE analysis showing presence of TZ on BMP-RA-TZ and BMP-GA-TZ surfaces. (B) TZ quantities, detected by enhanced BCA protein assay kit, similar for BMP-RA-TZ and BMP-GA-TZ (n=3). (C) Orientation of TZ (schematic) on BMP-RA-TZ and BMP-GA-TZ. (D) ELISA analysis of HER2-binding capacity of BMP-RA-TZ and BMP-GA-TZ (n=3), CK: no BMPs added. ***P <0.001; ****P <0.0001.