Fig 1: Transcript levels of selected junction proteins in endothelial cells co-cultured with astrocytes before, during and after oxygen-glucose deprivation (OGD) treatment or medium exchange.Endothelial cells which had undergone OGD (black bars) or medium exchange (gray bars) were collected for mRNA isolation. After the reverse transcription of the mRNA, the resultant cDNA was quantified by RT-qPCR using primers specifically designed for bovine. The mRNA amounts of claudin-1 (A), claudin-5 (B), claudin-12 (C), occludin (D), ZO-1 (E), ZO-2 (F), tricellulin (G), marveld3 (H) and PECAM-1 (I) were normalized to reference genes (for calculation see “Materials and methods” section). Bar graphs represent means and error bars are +SEM. The white bars show the values at t0, the gray bars show the cells subjected to medium exchange, while the black bars show the OGD treated cells. Columns were compared to t0 using one-way ANOVA and Dunnett’s multiple comparison post-test. *: p<0.05, **: p<0.01, ***: p<0.001. Each pair of columns were compared using one-way ANOVA with Bonferroni’s post-test.