Fig 1: The LINC complex exerts control over the transcriptome.(A) Inducible perturbation of the LINC-complex by SUN1L-KDEL. NIH 3T3 TetON cells were induced to express either SS-GFP-KDEL (control, KDEL) or SS-HA-SUN1L-KDEL (SUN1L) by the addition of doxycycline (+Dox). Untreated cells received no doxycycline (−Dox). When expressed, only the SUN1L but not the KDEL control (both green) led to loss of nesprin-3 (red) from the nuclear envelope. DNA is labeled with Hoechst dye (blue) in the merged image. Bar, 30 μm. (B) Volcano plots of statistical significance versus fold change between KDEL and SUN1L cells on 1-kPa (left) and 308-kPa (right) substrates; significantly differentially expressed genes are shown in red (downregulated) and green (upregulated). The horizontal line indicates the significance threshold, p = 0.05.