Fig 1: CELF2 overexpression inhibits tumor growth and angiogenesis. (A) Mice and tumor in the three groups. (B) Mouse weight changed from day 0 to day 21. ***P<0.001 vs. Con group. ##P<0.01 and ###P<0.001 vs. NC group. (C) Tumor volume changed from day 0 to day 21. **P<0.01 and ***P<0.001 vs. Con group. #P<0.05 and ###P<0.001 vs. NC group. (D) Tumor weight at day 21. ***P<0.001 vs. Con group. ###P<0.001 vs. NC group. (E) Tumor volume at day 21. ***P<0.001 vs. Con group. ###P<0.001 vs. NC group. (F) Angiogenesis was analyzed by HUVEC tube formation assay; scale bar, 100 µm. (G) Western blot analysis was performed to detect the expression of proteins associated with invasion and angiogenesis and NFATc1 in tumor tissues. **P<0.01 and ***P<0.001 vs. Con group. ##P<0.01 and ###P<0.001 vs. NC group. CELF2, CUGBP Elav-like family member 2; NFATc1, nuclear factor of activated T cells 1; Con, control; NC, negative control.
Fig 2: CELF2 overexpression suppresses HUVEC tube formation by downregulating NFATc1. (A) Cell Counting Kit-8 assay was used to examine the viability of HUVECs transfected with OverExp-CELF2 and OverExp-NFATc1. *P<0.05 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆P<0.01 vs. OverExp-CELF2 + vector group. (B) HUVEC tube formation assay was used to examine the angiogenic ability of HUVECs transfected with OverExp-CELF2 and OverExp-NFATc1; scale bar, 100 µm. (C) Western blot analysis was used to examine the expression of proteins associated with angiogenesis and NFATc1 in transfected cells. *P<0.05 and ***P<0.001 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆∆P<0.001 vs. OverExp-CELF2 + vector group. CELF2, CUGBP Elav-like family member 2; NFATc1, nuclear factor of activated T cells 1; Con, control; NC, negative control.
Fig 3: Association of CELF2 with NFATc1. (A) The association of CELF2 and NFATc1 was confirmed by RNA pull-down assay. (B) mRNA expression of NFATc1 in MCF-7 cells transfected with OverExp-NFATc1-#1/2 was examined by reverse transcription-quantitative PCR analysis. ***P<0.001 vs. Con group. ###P<0.001 vs. NC group. ∆∆∆P<0.001 vs. OverExp-NFATc1-#1 group. CELF2, CUGBP Elav-like family member 2; NFATc1, nuclear factor of activated T cells 1; Con, control; NC, negative control.
Fig 4: CELF2 overexpression suppresses viability and invasion of breast cancer cells by downregulating NFATc1. (A) Cell Counting Kit-8 assay was used to examine the viability of MCF-7 cells transfected with OverExp-CELF2 and OverExp-NFATc1. ***P<0.001 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆∆P<0.001 vs. OverExp-CELF2 + vector group. (B and C) Transwell assay was used to examine the invasion of MCF-7 cells transfected with OverExp-CELF2 and OverExp-NFATc1; scale bar, 100 µm. *P<0.05 and ***P<0.001 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆∆P<0.001 vs. OverExp-CELF2 + vector group. (D) Western blot analysis was used to examine the expression of proteins associated with invasion and NFATc1 in transfected cells. *P<0.05, **P<0.01 and ***P<0.001 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆∆P<0.001 vs. OverExp-CELF2 + vector group. CELF2, CUGBP Elav-like family member 2; NFATc1, nuclear factor of activated T cells 1; Con, control; NC, negative control.
Supplier Page from Abcam for Anti-NFAT2 antibody